LOCUS       BBa_J50035              1086 bp    DNA     linear       23-Dec-2024
DEFINITION  GFP Regulated by lactose/IPTG
ACCESSION   BBa_J50035
VERSION     BBa_J50035.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..949
                     /label=GFP protein
     CDS             230..949
                     /label=BBa_E0040
     stem_loop       969..1012
                     /label=stem_loop
     misc_feature    958..1037
                     /label=BBa_B0010
     terminator      958..1037
                     /label=BBa_B0010
     stem_loop       1053..1072
                     /label=T7 TE
     misc_feature    1079..1079
                     /label=stop
     polyA_site      1073..1086
                     /label=polya
     misc_feature    1046..1086
                     /label=BBa_B0012
     terminator      1046..1086
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcgtaaagg
      241 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      301 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      361 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      421 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      481 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      541 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      601 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      661 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      721 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      781 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      841 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      901 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca
      961 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1021 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1081 tttata
//