BBa_J52030 1 BBa_J52030 dnMyD88-GFP-PEST191 2006-10-14T11:00:00Z 2015-08-31T03:54:04Z dnMyD88- GFP- PEST191- dnMyD88-linker-GFP-linker-PEST191 is a part where dnMyD88 (important protein in signaling cascade through TLR4 and TLR5 receptor) is linked to GFP (reporter) and PEST191 sequence that leads towards degradation of protein linked with this sequence. It's estimated half-time is 2h. Both linkers have the same sequence and are 6 aminoacid long protein linker. true false _80_ 0 855 80 Discontinued false Part made by PCR overlap extension. Has mutations and may not be working. false Jelka Pohar annotation1903023 1 PEST191-degradation tag range1903023 1 1171 1656 annotation1903018 1 dnMyD88-GFP-PEST191 range1903018 1 1 1656 annotation1903019 1 dnMyD88 range1903019 1 1 417 annotation1903020 1 linker range1903020 1 418 435 annotation1903022 1 linker range1903022 1 1153 1170 annotation1903021 1 GFP range1903021 1 436 1153 BBa_J52030_sequence 1 atggcacgtttcgatgccttcatctgctattgccccagcgacatccagtttgtgcaggagatgatccggcaactggaacagacaaactatcgactgaagttgtgtgtgtctgaccgcgatgtcctgcctggcacctgtgtctggtctattgctagtgagctcatcgaaaagaggtgccgccggatggtggtggttgtctctgatgattacctccagagcaaggaatgtgacttccagaccaaatttgcactcagcctctctccaggtgcccatcagaagcgactgatccccatcaagtacaaggcaatgaagaaagagttccccagcatcctgaggttcatcactgtctgcgactacaccaacccctgcaccaaatcttggttctggactcgccttgccaaggccttgtccctgcccggtggttctggtggtactatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcccggtcgagctggacggcgacgtaaacggccacaagttcggcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacgccatcagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccctgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgatctgtacaagggtggttctggtggtactgctgcggtgctgaccttggccgtgctcttcctgacggggagccaggctcggcatttctggcagcaagatgaacccccccagagcccctgggatcgagtgaaggacctggccactgtgtacgtggatgtgctcaaagacagcggcagagactatgtgtcccagtttgaaggctccgccttgggaaaacagctaaacctaaagctccttgacaactgggacagcgtgacctccaccttcagcaagctgcgcgaacagctcggccctgtgacccaggagttctgggataacctggaaaaggagacagagggcctgaggcaggagatgagcaaggatctggaggaggtgaaggccaaggtgcagccctacctggacgacttccagaagaagtggcaggaggagatggagctctaccgccagaaggtggagccgctgcgcgcagagctccaagagggcgcgcgccagaagctgcacgagctgcgagagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z