LOCUS       BBa_J52031              2476 bp    DNA     linear       23-Dec-2024
DEFINITION  NF-kappaB dependent promoter + dnMyD88-linker-GFP-linker-PEST191
ACCESSION   BBa_J52031
VERSION     BBa_J52031.1  
FEATURES             Location/Qualifiers
     promoter        22..814
                     /label=NFkappaB
     misc_feature    1..814
                     /label=BioBrick
     primer_bind     813..855
                     /label=SI42
     primer_bind     16..43
                     /label=SI41
     promoter        1..814
                     /label=BBa_J52010
     misc_feature    821..2476
                     /label=dnMyD88-GFP-PEST191
     CDS             821..1237
                     /label=dnMyD88
     CDS             1238..1255
                     /label=linker
     CDS             1256..1973
                     /label=GFP
     CDS             1973..1990
                     /label=linker
     misc_feature    1991..2476
                     /label=PEST191-degradation tag
     CDS             821..2476
                     /label=BBa_J52030
ORIGIN
        1 ccaaagtggt gggattacag gcgtgagcca ctgtgcctgg cctccttttt atttttttca
       61 ctgaacaaac catgaaactt tcccagatgt aaatatctat ttcccatttt tcttttttta
      121 aaataaggca ttattttaac catttgagtg ttagatatta tttttagata atattttaat
      181 ttaggcataa ctgccgtgca aaatctgaag attaatatct accttgtgag tcattcctct
      241 gtgagacagt gcatgttaaa tatgttgaat tggcaggtga aaaaggaaga aaaaatgagt
      301 agtgattggt tatccacagc tatgaatgag aaattgaagg tagtagacta tggatgacaa
      361 acctattctt ggtttccttc tgtttctgaa attctaatta ctaccacaac tacatgagag
      421 acactactaa caagcaaagt tttacaactt tttaaagaca tagactttat gttattataa
      481 ttaaaaatca tgcatttttg tcatattaat aaaattgcat atacgatata aaggcatgga
      541 caaaggtgaa gtagcttcaa gagacagagt ttctgacatc attgtaattt taagcatcgt
      601 ggatattccc gggaaagttt ttggatgcca ttggggattt cctctttact ggatgtggac
      661 aatatcctcc tattattcac aggaagcggc ggccgccata aaagggcctc agccgaagta
      721 gtgttcagct gttcttggct gacttcacat caaaactcct atactgacct gagacagatc
      781 taagtaagct tggcattccg gtactgttgg taaatactag atggcacgtt tcgatgcctt
      841 catctgctat tgccccagcg acatccagtt tgtgcaggag atgatccggc aactggaaca
      901 gacaaactat cgactgaagt tgtgtgtgtc tgaccgcgat gtcctgcctg gcacctgtgt
      961 ctggtctatt gctagtgagc tcatcgaaaa gaggtgccgc cggatggtgg tggttgtctc
     1021 tgatgattac ctccagagca aggaatgtga cttccagacc aaatttgcac tcagcctctc
     1081 tccaggtgcc catcagaagc gactgatccc catcaagtac aaggcaatga agaaagagtt
     1141 ccccagcatc ctgaggttca tcactgtctg cgactacacc aacccctgca ccaaatcttg
     1201 gttctggact cgccttgcca aggccttgtc cctgcccggt ggttctggtg gtactatggt
     1261 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ccggtcgagc tggacggcga
     1321 cgtaaacggc cacaagttcg gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1381 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1441 gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     1501 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1561 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1621 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1681 ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     1741 caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1801 ctaccagcag aacaccccca tcggcgacgg ccctgtgctg ctgcccgaca accactacct
     1861 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1921 ggagttcgtg accgccgccg ggatcactct cggcatggac gatctgtaca agggtggttc
     1981 tggtggtact gctgcggtgc tgaccttggc cgtgctcttc ctgacgggga gccaggctcg
     2041 gcatttctgg cagcaagatg aaccccccca gagcccctgg gatcgagtga aggacctggc
     2101 cactgtgtac gtggatgtgc tcaaagacag cggcagagac tatgtgtccc agtttgaagg
     2161 ctccgccttg ggaaaacagc taaacctaaa gctccttgac aactgggaca gcgtgacctc
     2221 caccttcagc aagctgcgcg aacagctcgg ccctgtgacc caggagttct gggataacct
     2281 ggaaaaggag acagagggcc tgaggcagga gatgagcaag gatctggagg aggtgaaggc
     2341 caaggtgcag ccctacctgg acgacttcca gaagaagtgg caggaggaga tggagctcta
     2401 ccgccagaag gtggagccgc tgcgcgcaga gctccaagag ggcgcgcgcc agaagctgca
     2461 cgagctgcga gagtag
//