BBa_J52031 1 BBa_J52031 NF-kappaB dependent promoter + dnMyD88-linker-GFP-linker-PEST191 2006-10-14T11:00:00Z 2015-08-31T03:54:04Z NFkB - dnMyD88-linker-GFP-linker-PEST191 - already described (see BBa_J52030) This composite part is made of inducible promotor NFkB and dnMyD88 linked to reporter (GFP) and degradation tag. For use in human cells. true false _80_ 0 855 80 Discontinued false Promoter has a NotI restriction site. We did not alter DNA sequence, because this site did not affect our part construction and function of promoter. Has mutations and may not work. false Jelka Pohar component1903087 1 BBa_J52030 component1903080 1 BBa_J52010 annotation1903080 1 BBa_J52010 range1903080 1 1 814 annotation1903087 1 BBa_J52030 range1903087 1 821 2476 BBa_J52030 1 BBa_J52030 dnMyD88-GFP-PEST191 2006-10-14T11:00:00Z 2015-08-31T03:54:04Z dnMyD88- GFP- PEST191- dnMyD88-linker-GFP-linker-PEST191 is a part where dnMyD88 (important protein in signaling cascade through TLR4 and TLR5 receptor) is linked to GFP (reporter) and PEST191 sequence that leads towards degradation of protein linked with this sequence. It's estimated half-time is 2h. Both linkers have the same sequence and are 6 aminoacid long protein linker. true false _80_ 0 855 80 Discontinued false Part made by PCR overlap extension. Has mutations and may not be working. false Jelka Pohar annotation1903018 1 dnMyD88-GFP-PEST191 range1903018 1 1 1656 annotation1903023 1 PEST191-degradation tag range1903023 1 1171 1656 annotation1903021 1 GFP range1903021 1 436 1153 annotation1903019 1 dnMyD88 range1903019 1 1 417 annotation1903022 1 linker range1903022 1 1153 1170 annotation1903020 1 linker range1903020 1 418 435 BBa_J52010 1 NFkB NFkappaB-dependent promoter 2006-08-16T11:00:00Z 2015-08-31T03:54:04Z This promotor is present in human genome Released HQ 2013 Recognition of this promotor for initiation of transcription depends on the presence of nuclear factor kappa B. false false _80_ 0 853 80 In stock true (later) true Rok Tkavc annotation1897059 1 BioBrick range1897059 1 1 814 annotation1897060 1 SI42 range1897060 1 813 855 annotation1897061 1 SI41 range1897061 1 16 43 annotation1897058 1 NFkappaB range1897058 1 22 814 BBa_J52010_sequence 1 ccaaagtggtgggattacaggcgtgagccactgtgcctggcctcctttttatttttttcactgaacaaaccatgaaactttcccagatgtaaatatctatttcccatttttctttttttaaaataaggcattattttaaccatttgagtgttagatattatttttagataatattttaatttaggcataactgccgtgcaaaatctgaagattaatatctaccttgtgagtcattcctctgtgagacagtgcatgttaaatatgttgaattggcaggtgaaaaaggaagaaaaaatgagtagtgattggttatccacagctatgaatgagaaattgaaggtagtagactatggatgacaaacctattcttggtttccttctgtttctgaaattctaattactaccacaactacatgagagacactactaacaagcaaagttttacaactttttaaagacatagactttatgttattataattaaaaatcatgcatttttgtcatattaataaaattgcatatacgatataaaggcatggacaaaggtgaagtagcttcaagagacagagtttctgacatcattgtaattttaagcatcgtggatattcccgggaaagtttttggatgccattggggatttcctctttactggatgtggacaatatcctcctattattcacaggaagcggcggccgccataaaagggcctcagccgaagtagtgttcagctgttcttggctgacttcacatcaaaactcctatactgacctgagacagatctaagtaagcttggcattccggtactgttggtaaa BBa_J52031_sequence 1 ccaaagtggtgggattacaggcgtgagccactgtgcctggcctcctttttatttttttcactgaacaaaccatgaaactttcccagatgtaaatatctatttcccatttttctttttttaaaataaggcattattttaaccatttgagtgttagatattatttttagataatattttaatttaggcataactgccgtgcaaaatctgaagattaatatctaccttgtgagtcattcctctgtgagacagtgcatgttaaatatgttgaattggcaggtgaaaaaggaagaaaaaatgagtagtgattggttatccacagctatgaatgagaaattgaaggtagtagactatggatgacaaacctattcttggtttccttctgtttctgaaattctaattactaccacaactacatgagagacactactaacaagcaaagttttacaactttttaaagacatagactttatgttattataattaaaaatcatgcatttttgtcatattaataaaattgcatatacgatataaaggcatggacaaaggtgaagtagcttcaagagacagagtttctgacatcattgtaattttaagcatcgtggatattcccgggaaagtttttggatgccattggggatttcctctttactggatgtggacaatatcctcctattattcacaggaagcggcggccgccataaaagggcctcagccgaagtagtgttcagctgttcttggctgacttcacatcaaaactcctatactgacctgagacagatctaagtaagcttggcattccggtactgttggtaaatactagatggcacgtttcgatgccttcatctgctattgccccagcgacatccagtttgtgcaggagatgatccggcaactggaacagacaaactatcgactgaagttgtgtgtgtctgaccgcgatgtcctgcctggcacctgtgtctggtctattgctagtgagctcatcgaaaagaggtgccgccggatggtggtggttgtctctgatgattacctccagagcaaggaatgtgacttccagaccaaatttgcactcagcctctctccaggtgcccatcagaagcgactgatccccatcaagtacaaggcaatgaagaaagagttccccagcatcctgaggttcatcactgtctgcgactacaccaacccctgcaccaaatcttggttctggactcgccttgccaaggccttgtccctgcccggtggttctggtggtactatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcccggtcgagctggacggcgacgtaaacggccacaagttcggcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacgccatcagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccctgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgatctgtacaagggtggttctggtggtactgctgcggtgctgaccttggccgtgctcttcctgacggggagccaggctcggcatttctggcagcaagatgaacccccccagagcccctgggatcgagtgaaggacctggccactgtgtacgtggatgtgctcaaagacagcggcagagactatgtgtcccagtttgaaggctccgccttgggaaaacagctaaacctaaagctccttgacaactgggacagcgtgacctccaccttcagcaagctgcgcgaacagctcggccctgtgacccaggagttctgggataacctggaaaaggagacagagggcctgaggcaggagatgagcaaggatctggaggaggtgaaggccaaggtgcagccctacctggacgacttccagaagaagtggcaggaggagatggagctctaccgccagaaggtggagccgctgcgcgcagagctccaagagggcgcgcgccagaagctgcacgagctgcgagagtag BBa_J52030_sequence 1 atggcacgtttcgatgccttcatctgctattgccccagcgacatccagtttgtgcaggagatgatccggcaactggaacagacaaactatcgactgaagttgtgtgtgtctgaccgcgatgtcctgcctggcacctgtgtctggtctattgctagtgagctcatcgaaaagaggtgccgccggatggtggtggttgtctctgatgattacctccagagcaaggaatgtgacttccagaccaaatttgcactcagcctctctccaggtgcccatcagaagcgactgatccccatcaagtacaaggcaatgaagaaagagttccccagcatcctgaggttcatcactgtctgcgactacaccaacccctgcaccaaatcttggttctggactcgccttgccaaggccttgtccctgcccggtggttctggtggtactatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcccggtcgagctggacggcgacgtaaacggccacaagttcggcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacgccatcagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccctgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgatctgtacaagggtggttctggtggtactgctgcggtgctgaccttggccgtgctcttcctgacggggagccaggctcggcatttctggcagcaagatgaacccccccagagcccctgggatcgagtgaaggacctggccactgtgtacgtggatgtgctcaaagacagcggcagagactatgtgtcccagtttgaaggctccgccttgggaaaacagctaaacctaaagctccttgacaactgggacagcgtgacctccaccttcagcaagctgcgcgaacagctcggccctgtgacccaggagttctgggataacctggaaaaggagacagagggcctgaggcaggagatgagcaaggatctggaggaggtgaaggccaaggtgcagccctacctggacgacttccagaagaagtggcaggaggagatggagctctaccgccagaaggtggagccgctgcgcgcagagctccaagagggcgcgcgccagaagctgcacgagctgcgagagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z