BBa_J54006
1
BBa_J54006
PoPS->deoR protein generator
2006-08-03T11:00:00Z
2015-08-31T03:54:05Z
This part is based on part BBa_J54000
This is a RBS, CDS,Terminator construct used to take PoPS signal and produce deoR protein.
false
false
_76_
0
766
76
Not in stock
false
None.
false
Shogo Hamada
component1893506
1
BBa_B0010
component1893508
1
BBa_B0012
component1893505
1
BBa_J54000
component1893500
1
BBa_B0030
annotation1893505
1
BBa_J54000
range1893505
1
22
783
annotation1893506
1
BBa_B0010
range1893506
1
792
871
annotation1893500
1
BBa_B0030
range1893500
1
1
15
annotation1893508
1
BBa_B0012
range1893508
1
880
920
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_J54000
1
BBa_J54000
deoR repressor protein
2006-08-03T11:00:00Z
2015-08-31T03:54:04Z
It comes from Escherichia Coli.
It is a negative DNA-binding transcriptional regulator that is involved in the negative regulation of the genes that encode the nucleotide catabolism (deoCABD, nupG, and tsx). The protein family is DeoR.
RegulonDB
http://regulondb.ccg.unam.mx:80/SearchListController?key_id_org=ECK12&object_id=DEOR&object_form=GN
false
false
_76_
0
766
76
Not in stock
false
I have not made any changes from wildtype.
false
Shogo Hamada
annotation1893460
1
double TAA stop codons
range1893460
1
757
762
annotation1893463
1
start codons
range1893463
1
1
3
annotation1893453
1
deoR
range1893453
1
1
762
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J54000_sequence
1
atggaaacacgtcgcgaagagcgtatcgggcagctgctgcaagaattaaaacgcagcgataagttacatcttaaagacgccgccgccctgcttggggtttcggagatgacgattcgtcgcgatctgaacaaccacagtgcgcccgtcgttttgctcggcggctatattgttctggaaccgcgcagtgccagccattacctgttaagcgatcaaaaatcccgcctggtggaagaaaaacgccgggcggcaaaactggctgcgacgctggtagaacccgatcagaccctcttttttgactgtggcaccaccacgccgtggattattgaagcgattgataatgaaatcccttttaccgccgtttgttattcgctaaatacctttctggcgctgaaagagaaaccccattgccgcgcgtttctttgcggtggtgaatttcacgccagcaacgccattttcaaacccatcgattttcagcaaacgctgaataatttttgcccggatatcgctttttattctgcggcgggcgtgcatgtcagtaaaggcgctacctgttttaatcttgaagagttgccggtaaaacactgggccatgtcgatggcgcaaaagcatgtgctggttgtcgaccacagtaaatttggcaaggtgcgtccggcgcgcatgggtgacctgaaacgctttgatattgtggtgagcgattgttgcccggaagatgagtatgtgaagtacgcgcagacgcagcgcattaagttgatgtattaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J54006_sequence
1
attaaagaggagaaatactagatggaaacacgtcgcgaagagcgtatcgggcagctgctgcaagaattaaaacgcagcgataagttacatcttaaagacgccgccgccctgcttggggtttcggagatgacgattcgtcgcgatctgaacaaccacagtgcgcccgtcgttttgctcggcggctatattgttctggaaccgcgcagtgccagccattacctgttaagcgatcaaaaatcccgcctggtggaagaaaaacgccgggcggcaaaactggctgcgacgctggtagaacccgatcagaccctcttttttgactgtggcaccaccacgccgtggattattgaagcgattgataatgaaatcccttttaccgccgtttgttattcgctaaatacctttctggcgctgaaagagaaaccccattgccgcgcgtttctttgcggtggtgaatttcacgccagcaacgccattttcaaacccatcgattttcagcaaacgctgaataatttttgcccggatatcgctttttattctgcggcgggcgtgcatgtcagtaaaggcgctacctgttttaatcttgaagagttgccggtaaaacactgggccatgtcgatggcgcaaaagcatgtgctggttgtcgaccacagtaaatttggcaaggtgcgtccggcgcgcatgggtgacctgaaacgctttgatattgtggtgagcgattgttgcccggaagatgagtatgtgaagtacgcgcagacgcagcgcattaagttgatgtattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z