##gff-version 3
##sequence-region BBa_J54007 1 1185
BBa_J54007	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J54007	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J54007	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J54007	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J54007	.	CDS	20	1048	.	+	0	ID=BBa_J54001;Name=BBa_J54001
BBa_J54007	.	stop_codon	1043	1048	.	+	0	ID=annotation1893458;Name=Double stop codon;Parent=BBa_J54001
BBa_J54007	.	CDS	20	1048	.	+	0	ID=annotation1893455;Name=cytR;Parent=BBa_J54001
BBa_J54007	.	point_mutation	20	20	.	+	0	ID=annotation1893462;Name=G to A;Parent=BBa_J54001
BBa_J54007	.	terminator	1057	1136	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J54007	.	stem_loop	1068	1111	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J54007	.	engineered_region	1057	1136	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J54007	.	terminator	1145	1185	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J54007	.	polyA_site	1172	1185	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J54007	.	engineered_region	1145	1185	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J54007	.	stem_loop	1152	1171	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J54007	.	stop_codon	1178	1178	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_J54007
tcacacaggaaagtactagatgaaagcgaagaagcaggaaactgccgcgaccatgaaagacgttgccctcaaggcaaaa
gtctctacagcgaccgtctcccgagcattaatgaatcccgataaagtctcccaggccacccgtaatcgggttgaaaaag
cggcccgggaagtgggttatttaccgcagcctatggggcgcaacgtcaagcgtaatgaatcccgcaccattctggtgat
tgtcccggatatctgcgatcccttctttagcgaaattattcgcggtatcgaagttacggcggcaaatcacggatatctg
gtgctgattggcgactgtgcgcatcaaaatcagcaggaaaaaacctttatcgatttgatcatcaccaagcaaattgatg
gcatgttgttgctgggttcaaggctgccgtttgatgccagcattgaggaacagcgtaatctgccgccgatggtgatggc
gaacgaatttgcaccggagctggagctgcctacagttcatatcgacaatctgaccgccgcatttgatgcagtaaattat
ttatatgagcaagggcataaacggattggctgtatagccggtcccgaagagatgccgctgtgtcactaccgcctgcaag
gctatgttcaggcgctgcgtcgctgcggcattatggttgatccgcaatacatcgcccgtggcgacttcaccttcgaagc
cggaagcaaagcgatgcagcagctgcttgatcttccacaaccgcctactgctgtcttctgccatagcgatgtgatggcg
ctcggcgcactttctcaggcaaaacgccaggggctgaaagtcccggaagacctttccataatcggttttgataacatcg
acctgacgcaattttgtgatccgccgctgacaaccatcgcgcagccgcgttacgaaatcggtcgggaagctatgctgtt
attgcttgatcaaatgcaggggcaacacgttggcagtggctctcgtttaatggactgcgaacttatcatccggggatca
acacgcgcgttaccttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata