BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_J54002 1 BBa_J54002 galS 2006-08-03T11:00:00Z 2015-08-31T03:54:05Z regulonDB It comes from E.coli K12. GalS is a isorepressor of galR. false false _76_ 0 765 76 Not in stock false I have not made mutation to wild type. false Shotaro Ayukawa annotation1893459 1 double TAA range1893459 1 1039 1044 annotation1893456 1 GalS range1893456 1 1 1044 BBa_J54009 1 BBa_J54009 PoPs -> GalS protein generator 2006-08-03T11:00:00Z 2015-08-31T03:54:05Z This device is based on BBa_J54002 (GalS) This is RBS, CDS, teminator consturuct used to PoPS signal and produce the protein GalS false false _76_ 0 765 76 Not in stock false none false Shotaro Ayukawa component1893478 1 BBa_B0034 component1893482 1 BBa_B0012 component1893481 1 BBa_J54002 component1893486 1 BBa_B0011 annotation1893486 1 BBa_B0011 range1893486 1 1120 1165 annotation1893482 1 BBa_B0012 range1893482 1 1071 1111 annotation1893481 1 BBa_J54002 range1893481 1 19 1062 annotation1893478 1 BBa_B0034 range1893478 1 1 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J54009_sequence 1 aaagaggagaaatactagatgatcaccattcgtgatgtagcgcgtcaggctggcgtctctgtggcaacggtttcccgggtgctcaataacagcacgctggtcagtgccgacacgcgtgaagcagtaatgaaagccgtgagtgagctggattatcggccaaacgccaatgcccaggcgctggcaactcaggttagcgacaccattggcgtggtggtgatggacgtttctgatgcgtttttcggcgcgctggtaaaagcggtggatctggtcgctcagcagcatcagaaatacgtgctaatcggcaatagctatcatgaagcggaaaaagagcgtcacgccattgaggtgttaattcgccagcgttgtaatgcgttgattgttcactcaaaagcattgagtgacgatgaactggcgcaatttatggataacattcccggtatggtgttaatcaaccgcgttgtgccggggtacgcccatcgttgcgtttgcctggataatctcagcggtgcccgaatggcgacgcgcatgttgctgaataacggtcatcaacgtattggttatctttcttccagccacggcattgaagatgacgccatgcgtaaagcaggctggatgagtgcgttgaaagagcaggatattattccgccggaaagctggattggcgctggtacgccggacatgccgggcggtgaggcggcgatggttgaactgctggggcgcaatctacaacttaccgctgtatttgcttataacgacaatatggccgctggcgcactgacagcattaaaagataatggcattgcgattccgttacatctctcaatcatcggtttcgatgatattcccatcgcccgttacaccgacccgcaattaacgaccgtgcgttatcccattgcttcaatggcgaaattagccaccgaactggccttgcagggggcagcaggcaatattgatcctcgtgccagccactgttttatgccgacgttagtgcgtcgccattctgtcgcaacacgccagaatgcggcggcgatcactaactcaacaaatcaggcgatgtaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0034_sequence 1 aaagaggagaaa BBa_J54002_sequence 1 atgatcaccattcgtgatgtagcgcgtcaggctggcgtctctgtggcaacggtttcccgggtgctcaataacagcacgctggtcagtgccgacacgcgtgaagcagtaatgaaagccgtgagtgagctggattatcggccaaacgccaatgcccaggcgctggcaactcaggttagcgacaccattggcgtggtggtgatggacgtttctgatgcgtttttcggcgcgctggtaaaagcggtggatctggtcgctcagcagcatcagaaatacgtgctaatcggcaatagctatcatgaagcggaaaaagagcgtcacgccattgaggtgttaattcgccagcgttgtaatgcgttgattgttcactcaaaagcattgagtgacgatgaactggcgcaatttatggataacattcccggtatggtgttaatcaaccgcgttgtgccggggtacgcccatcgttgcgtttgcctggataatctcagcggtgcccgaatggcgacgcgcatgttgctgaataacggtcatcaacgtattggttatctttcttccagccacggcattgaagatgacgccatgcgtaaagcaggctggatgagtgcgttgaaagagcaggatattattccgccggaaagctggattggcgctggtacgccggacatgccgggcggtgaggcggcgatggttgaactgctggggcgcaatctacaacttaccgctgtatttgcttataacgacaatatggccgctggcgcactgacagcattaaaagataatggcattgcgattccgttacatctctcaatcatcggtttcgatgatattcccatcgcccgttacaccgacccgcaattaacgaccgtgcgttatcccattgcttcaatggcgaaattagccaccgaactggccttgcagggggcagcaggcaatattgatcctcgtgccagccactgttttatgccgacgttagtgcgtcgccattctgtcgcaacacgccagaatgcggcggcgatcactaactcaacaaatcaggcgatgtaataa BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z