LOCUS       BBa_J54111              1005 bp    DNA     linear       25-Nov-2024
DEFINITION  MelR_regulated_promoter-GFP(A-1)
ACCESSION   BBa_J54111
VERSION     BBa_J54111.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    14..18
                     /label=BamHI
     misc_feature    19..87
                     /label=MelR_regulated promoter
     misc_feature    14..89
                     /label=BBa_J54110
     misc_feature    98..102
                     /label=BglII
     misc_feature    111..116
                     /label=MulI
     misc_feature    98..116
                     /label=BBa_J54025
     RBS             125..139
                     /label=RBS-1\Strong
     misc_feature    125..139
                     /label=BBa_B0030
     RBS             132..136
                     /label=RBS
     misc_feature    146..865
                     /label=BBa_E0040
     misc_feature    874..953
                     /label=BBa_B0010
     stem_loop       885..928
                     /label=stem_loop
     misc_feature    962..1002
                     /label=BBa_B0012
     stem_loop       969..988
                     /label=T7 TE
     polyA_site      989..1002
                     /label=polya
     misc_feature    995..995
                     /label=stop
ORIGIN
        1 gtcgacatgc gcggatccat ctgagtttat ggaatgccat tacctgaaaa gcagaggttt
       61 tctgcggatt cccctgccat gttgaagttg atcctgtcgg agatctgtcg gaaaacgcgt
      121 actagagatt aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt
      181 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      241 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
      301 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt
      361 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg
      421 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
      481 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
      541 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
      601 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
      661 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
      721 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
      781 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
      841 tggcatggat gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//