LOCUS       BBa_J54121               972 bp    DNA     linear       13-Jun-2024
DEFINITION  EmrR_regulated promoter(A-2)
ACCESSION   BBa_J54121
VERSION     BBa_J54121.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    14..18
                     /label=BamHI
     misc_feature    19..56
                     /label=BetI_regulated promoter
     promoter        14..59
                     /label=BBa_J54120
     misc_feature    68..72
                     /label=BglII
     misc_feature    81..86
                     /label=MulI
     misc_feature    68..86
                     /label=BBa_J54025
     RBS             95..109
                     /label=RBS-1\Strong
     RBS             102..106
                     /label=RBS
     misc_feature    95..109
                     /label=BBa_B0030
     RBS             95..109
                     /label=BBa_B0030
     CDS             116..835
                     /label=GFP protein
     CDS             116..835
                     /label=BBa_E0040
     stem_loop       855..898
                     /label=stem_loop
     misc_feature    844..923
                     /label=BBa_B0010
     terminator      844..923
                     /label=BBa_B0010
     stem_loop       939..958
                     /label=T7 TE
     misc_feature    965..965
                     /label=stop
     polyA_site      959..972
                     /label=polya
     misc_feature    932..972
                     /label=BBa_B0012
     terminator      932..972
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gaggatccac atttacttta tttgtcactg tcgttactat atcggctgct
       61 actagaggat ctgtcggata acgcgttact agagattaaa gaggagaaat actagatgcg
      121 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      181 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      241 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      301 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
      361 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      421 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      481 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
      541 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
      601 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
      661 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
      721 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
      781 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
      841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      961 tctgcgttta ta
//