LOCUS       BBa_J54122              1000 bp    DNA     linear       29-May-2024
DEFINITION  AND GATE"salicilate and oleate"(A-21)
ACCESSION   BBa_J54122
VERSION     BBa_J54122.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    14..18
                     /label=BamHI
     misc_feature    19..56
                     /label=BetI_regulated promoter
     promoter        14..59
                     /label=BBa_J54120
     misc_feature    89..95
                     /label=BglII
     misc_feature    73..88
                     /label=FadR
     promoter        68..101
                     /label=BBa_J54220
     misc_feature    110..114
                     /label=MulI
     misc_feature    110..114
                     /label=BBa_J54034
     RBS             123..137
                     /label=RBS-1\Strong
     RBS             130..134
                     /label=RBS
     misc_feature    123..137
                     /label=BBa_B0030
     RBS             123..137
                     /label=BBa_B0030
     CDS             144..863
                     /label=GFP protein
     CDS             144..863
                     /label=BBa_E0040
     stem_loop       883..926
                     /label=stem_loop
     misc_feature    872..951
                     /label=BBa_B0010
     terminator      872..951
                     /label=BBa_B0010
     stem_loop       967..986
                     /label=T7 TE
     misc_feature    993..993
                     /label=stop
     polyA_site      987..1000
                     /label=polya
     misc_feature    960..1000
                     /label=BBa_B0012
     terminator      960..1000
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gaggatccac atttacttta tttgtcactg tcgttactat atcggctgct
       61 actagaggat catctggtac gaccagatag atctatgcat atactagagc gcgttactag
      121 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      181 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      241 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      301 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      361 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      421 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      481 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      541 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      601 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      661 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      721 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      781 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      841 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata
//