LOCUS       BBa_J54140              2098 bp    DNA     linear       22-Dec-2024
DEFINITION  LuxR_regulated(A-4)
ACCESSION   BBa_J54140
VERSION     BBa_J54140.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1167..1171
                     /label=SalI
     misc_feature    1181..1185
                     /label=BamHI
     misc_feature    1167..1185
                     /label=BBa_J54033
     misc_feature    1194..1198
                     /label=BglII
     misc_feature    1207..1212
                     /label=MulI
     misc_feature    1194..1212
                     /label=BBa_J54025
     RBS             1221..1235
                     /label=RBS-1\Strong
     RBS             1228..1232
                     /label=RBS
     misc_feature    1221..1235
                     /label=BBa_B0030
     RBS             1221..1235
                     /label=BBa_B0030
     CDS             1242..1961
                     /label=GFP protein
     CDS             1242..1961
                     /label=BBa_E0040
     stem_loop       1981..2024
                     /label=stem_loop
     misc_feature    1970..2049
                     /label=BBa_B0010
     terminator      1970..2049
                     /label=BBa_B0010
     stem_loop       2065..2084
                     /label=T7 TE
     misc_feature    2091..2091
                     /label=stop
     polyA_site      2085..2098
                     /label=polya
     misc_feature    2058..2098
                     /label=BBa_B0012
     terminator      2058..2098
                     /label=BBa_B0012
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagaggtcg actgacgact ggatctacta gaggatctgt
     1201 cggataacgc gttactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac
     1261 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     1321 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     1381 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
     1441 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     1501 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     1561 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     1621 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     1681 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     1741 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1801 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1861 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1921 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
     1981 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2041 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//