LOCUS       BBa_J54142              2130 bp    DNA     linear       23-Dec-2024
DEFINITION  AND GATE"AHL and aTc"
ACCESSION   BBa_J54142
VERSION     BBa_J54142.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1167..1171
                     /label=SalI
     misc_feature    1181..1185
                     /label=BamHI
     misc_feature    1167..1185
                     /label=BBa_J54033
     misc_feature    1219..1225
                     /label=BglII
     misc_feature    1194..1218
                     /label=TetR
     promoter        1194..1231
                     /label=BBa_J54230
     misc_feature    1240..1244
                     /label=MulI
     misc_feature    1240..1244
                     /label=BBa_J54034
     RBS             1253..1267
                     /label=RBS-1\Strong
     RBS             1260..1264
                     /label=RBS
     misc_feature    1253..1267
                     /label=BBa_B0030
     RBS             1253..1267
                     /label=BBa_B0030
     CDS             1274..1993
                     /label=GFP protein
     CDS             1274..1993
                     /label=BBa_E0040
     stem_loop       2013..2056
                     /label=stem_loop
     misc_feature    2002..2081
                     /label=BBa_B0010
     terminator      2002..2081
                     /label=BBa_B0010
     stem_loop       2097..2116
                     /label=T7 TE
     misc_feature    2123..2123
                     /label=stop
     polyA_site      2117..2130
                     /label=polya
     misc_feature    2090..2130
                     /label=BBa_B0012
     terminator      2090..2130
                     /label=BBa_B0012
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagaggtcg actgacgact ggatctacta gaggatcgtc
     1201 cctatcagtg atagagatag atctatcgta atactagagc gcgttactag agattaaaga
     1261 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1321 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1381 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1441 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1501 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1561 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1621 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1681 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1741 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1801 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1861 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1921 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1981 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2041 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2101 caccttcggg tgggcctttc tgcgtttata
//