LOCUS       BBa_J54150              2150 bp    DNA     linear       22-Dec-2024
DEFINITION  AraC_regulated(A-5)
ACCESSION   BBa_J54150
VERSION     BBa_J54150.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1219..1223
                     /label=SalI
     misc_feature    1233..1237
                     /label=BamHI
     misc_feature    1219..1237
                     /label=BBa_J54033
     misc_feature    1246..1250
                     /label=BglII
     misc_feature    1259..1264
                     /label=MulI
     misc_feature    1246..1264
                     /label=BBa_J54025
     RBS             1273..1287
                     /label=RBS-1\Strong
     RBS             1280..1284
                     /label=RBS
     misc_feature    1273..1287
                     /label=BBa_B0030
     RBS             1273..1287
                     /label=BBa_B0030
     CDS             1294..2013
                     /label=GFP protein
     CDS             1294..2013
                     /label=BBa_E0040
     stem_loop       2033..2076
                     /label=stem_loop
     misc_feature    2022..2101
                     /label=BBa_B0010
     terminator      2022..2101
                     /label=BBa_B0010
     stem_loop       2117..2136
                     /label=T7 TE
     misc_feature    2143..2143
                     /label=stop
     polyA_site      2137..2150
                     /label=polya
     misc_feature    2110..2150
                     /label=BBa_B0012
     terminator      2110..2150
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagaggt cgactgacga ctggatctac tagaggatct gtcggataac
     1261 gcgttactag agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact
     1321 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     1381 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     1441 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
     1501 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
     1561 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
     1621 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
     1681 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
     1741 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
     1801 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     1861 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     1921 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     1981 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg
     2041 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2101 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//