LOCUS       BBa_J54153              2182 bp    DNA     linear       26-Nov-2024
DEFINITION  AND GATE"Arabinose and aTc"
ACCESSION   BBa_J54153
VERSION     BBa_J54153.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1219..1223
                     /label=SalI
     misc_feature    1233..1237
                     /label=BamHI
     misc_feature    1219..1237
                     /label=BBa_J54033
     misc_feature    1271..1277
                     /label=BglII
     misc_feature    1246..1270
                     /label=TetR
     promoter        1246..1283
                     /label=BBa_J54230
     misc_feature    1292..1296
                     /label=MulI
     misc_feature    1292..1296
                     /label=BBa_J54034
     RBS             1305..1319
                     /label=RBS-1\Strong
     RBS             1312..1316
                     /label=RBS
     misc_feature    1305..1319
                     /label=BBa_B0030
     RBS             1305..1319
                     /label=BBa_B0030
     CDS             1326..2045
                     /label=GFP protein
     CDS             1326..2045
                     /label=BBa_E0040
     stem_loop       2065..2108
                     /label=stem_loop
     misc_feature    2054..2133
                     /label=BBa_B0010
     terminator      2054..2133
                     /label=BBa_B0010
     stem_loop       2149..2168
                     /label=T7 TE
     misc_feature    2175..2175
                     /label=stop
     polyA_site      2169..2182
                     /label=polya
     misc_feature    2142..2182
                     /label=BBa_B0012
     terminator      2142..2182
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagaggt cgactgacga ctggatctac tagaggatcg tccctatcag
     1261 tgatagagat agatctatcg taatactaga gcgcgttact agagattaaa gaggagaaat
     1321 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     1381 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     1441 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
     1501 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
     1561 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     1621 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
     1681 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     1741 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
     1801 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
     1861 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     1921 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
     1981 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     2041 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     2101 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     2161 ggtgggcctt tctgcgttta ta
//