LOCUS       BBa_J54202               976 bp    DNA     linear       08-Jun-2024
DEFINITION  lacq_Promoter-GFP(B)
ACCESSION   BBa_J54202
VERSION     BBa_J54202.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     promoter        14..63
                     /label=BBa_J54200
     misc_feature    72..76
                     /label=BglII
     misc_feature    85..90
                     /label=MulI
     misc_feature    72..90
                     /label=BBa_J54025
     RBS             99..113
                     /label=RBS-1\Strong
     RBS             106..110
                     /label=RBS
     misc_feature    99..113
                     /label=BBa_B0030
     RBS             99..113
                     /label=BBa_B0030
     CDS             120..839
                     /label=GFP protein
     CDS             120..839
                     /label=BBa_E0040
     stem_loop       859..902
                     /label=stem_loop
     misc_feature    848..927
                     /label=BBa_B0010
     terminator      848..927
                     /label=BBa_B0010
     stem_loop       943..962
                     /label=T7 TE
     misc_feature    969..969
                     /label=stop
     polyA_site      963..976
                     /label=polya
     misc_feature    936..976
                     /label=BBa_B0012
     terminator      936..976
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gagccgtgac ggatcctggt gcaaaacctt tcgcggtatg gcatgatagc
       61 gcctactaga ggatctgtcg gataacgcgt tactagagat taaagaggag aaatactaga
      121 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      181 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      241 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      301 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
      361 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      421 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
      481 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      541 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      601 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
      661 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
      721 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
      781 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
      841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      961 cctttctgcg tttata
//