##gff-version 3
##sequence-region BBa_J54202 1 976
BBa_J54202	.	tag	1	5	.	+	0	ID=BBa_J54023;Name=BBa_J54023
BBa_J54202	.	tag	1	5	.	+	0	ID=annotation1903320;Name=Sal;Parent=BBa_J54023
BBa_J54202	.	tag	72	90	.	+	0	ID=BBa_J54025;Name=BBa_J54025
BBa_J54202	.	tag	72	76	.	+	0	ID=annotation1903321;Name=BglII;Parent=BBa_J54025
BBa_J54202	.	tag	85	90	.	+	0	ID=annotation1903322;Name=MulI;Parent=BBa_J54025
BBa_J54202	.	ribosome_entry_site	99	113	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J54202	.	engineered_region	99	113	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J54202	.	ribosome_entry_site	106	110	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J54202	.	ribosome_entry_site	99	113	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J54202	.	CDS	120	839	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J54202	.	CDS	120	839	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J54202	.	terminator	848	927	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J54202	.	engineered_region	848	927	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J54202	.	stem_loop	859	902	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J54202	.	terminator	936	976	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J54202	.	engineered_region	936	976	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J54202	.	polyA_site	963	976	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J54202	.	stem_loop	943	962	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J54202	.	stop_codon	969	969	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J54202	.	promoter	14	63	.	+	0	ID=BBa_J54200;Name=BBa_J54200
BBa_J54202	.	tag	20	26	.	+	0	ID=annotation1903214;Name=BamHI;Parent=BBa_J54200
BBa_J54202	.	promoter	27	61	.	+	0	ID=annotation1903215;Name=lacq Promoter;Parent=BBa_J54200
>BBa_J54202
gtcgatactagagccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggatctgtc
ggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata