LOCUS       BBa_J54212              1007 bp    DNA     linear       05-Dec-2025
DEFINITION  RbsR_regulated(B-1)
ACCESSION   BBa_J54212
VERSION     BBa_J54212.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     promoter        14..63
                     /label=BBa_J54200
     misc_feature    96..102
                     /label=BglII
     misc_feature    77..95
                     /label=RbsR_Binding_Site
     promoter        72..108
                     /label=BBa_J54210
     misc_feature    117..121
                     /label=MulI
     misc_feature    117..121
                     /label=BBa_J54034
     RBS             130..144
                     /label=RBS-1\Strong
     RBS             137..141
                     /label=RBS
     misc_feature    130..144
                     /label=BBa_B0030
     RBS             130..144
                     /label=BBa_B0030
     CDS             151..870
                     /label=GFP protein
     CDS             151..870
                     /label=BBa_E0040
     stem_loop       890..933
                     /label=stem_loop
     misc_feature    879..958
                     /label=BBa_B0010
     terminator      879..958
                     /label=BBa_B0010
     stem_loop       974..993
                     /label=T7 TE
     misc_feature    1000..1000
                     /label=stop
     polyA_site      994..1007
                     /label=polya
     misc_feature    967..1007
                     /label=BBa_B0012
     terminator      967..1007
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gagccgtgac ggatcctggt gcaaaacctt tcgcggtatg gcatgatagc
       61 gcctactaga ggatcgtcag cgaaacgttt cgctgagatc tatgcggata ctagagcgcg
      121 ttactagaga ttaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
      181 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      241 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      301 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      361 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      421 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      481 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      541 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      601 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      661 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      721 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      781 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      841 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
      901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      961 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//