##gff-version 3
##sequence-region BBa_J54212 1 1007
BBa_J54212	.	promoter	72	108	.	+	0	ID=BBa_J54210;Name=BBa_J54210
BBa_J54212	.	operator	77	95	.	+	0	ID=annotation1903232;Name=RbsR_Binding_Site;Parent=BBa_J54210
BBa_J54212	.	tag	96	102	.	+	0	ID=annotation1903231;Name=BglII;Parent=BBa_J54210
BBa_J54212	.	tag	117	121	.	+	0	ID=BBa_J54034;Name=BBa_J54034
BBa_J54212	.	tag	117	121	.	+	0	ID=annotation1903325;Name=MulI;Parent=BBa_J54034
BBa_J54212	.	ribosome_entry_site	130	144	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J54212	.	ribosome_entry_site	130	144	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J54212	.	ribosome_entry_site	137	141	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J54212	.	engineered_region	130	144	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J54212	.	promoter	14	63	.	+	0	ID=BBa_J54200;Name=BBa_J54200
BBa_J54212	.	tag	20	26	.	+	0	ID=annotation1903214;Name=BamHI;Parent=BBa_J54200
BBa_J54212	.	promoter	27	61	.	+	0	ID=annotation1903215;Name=lacq Promoter;Parent=BBa_J54200
BBa_J54212	.	terminator	879	958	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J54212	.	stem_loop	890	933	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J54212	.	engineered_region	879	958	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J54212	.	tag	1	5	.	+	0	ID=BBa_J54023;Name=BBa_J54023
BBa_J54212	.	tag	1	5	.	+	0	ID=annotation1903320;Name=Sal;Parent=BBa_J54023
BBa_J54212	.	CDS	151	870	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J54212	.	CDS	151	870	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J54212	.	terminator	967	1007	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J54212	.	stop_codon	1000	1000	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J54212	.	engineered_region	967	1007	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J54212	.	stem_loop	974	993	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J54212	.	polyA_site	994	1007	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_J54212
gtcgatactagagccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggatcgtca
gcgaaacgtttcgctgagatctatgcggatactagagcgcgttactagagattaaagaggagaaatactagatgcgtaa
aggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtc
agtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttc
catggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatga
ctttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagaca
cgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaa
acattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaat
caaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcca
attggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaa
agagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata