LOCUS       BBa_J54213              1052 bp    DNA     linear       01-May-2024
DEFINITION  RbsRtandem_regulated(B-1-1)
ACCESSION   BBa_J54213
VERSION     BBa_J54213.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     promoter        14..63
                     /label=BBa_J54200
     misc_feature    96..102
                     /label=BglII
     misc_feature    77..95
                     /label=RbsR_Binding_Site
     promoter        72..108
                     /label=BBa_J54210
     misc_feature    141..147
                     /label=BglII
     misc_feature    122..140
                     /label=RbsR_Binding_Site
     promoter        117..153
                     /label=BBa_J54210
     misc_feature    162..166
                     /label=MulI
     misc_feature    162..166
                     /label=BBa_J54034
     RBS             175..189
                     /label=RBS-1\Strong
     RBS             182..186
                     /label=RBS
     misc_feature    175..189
                     /label=BBa_B0030
     RBS             175..189
                     /label=BBa_B0030
     CDS             196..915
                     /label=GFP protein
     CDS             196..915
                     /label=BBa_E0040
     stem_loop       935..978
                     /label=stem_loop
     misc_feature    924..1003
                     /label=BBa_B0010
     terminator      924..1003
                     /label=BBa_B0010
     stem_loop       1019..1038
                     /label=T7 TE
     misc_feature    1045..1045
                     /label=stop
     polyA_site      1039..1052
                     /label=polya
     misc_feature    1012..1052
                     /label=BBa_B0012
     terminator      1012..1052
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gagccgtgac ggatcctggt gcaaaacctt tcgcggtatg gcatgatagc
       61 gcctactaga ggatcgtcag cgaaacgttt cgctgagatc tatgcggata ctagaggatc
      121 gtcagcgaaa cgtttcgctg agatctatgc ggatactaga gcgcgttact agagattaaa
      181 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      241 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      301 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      361 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      421 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      481 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      541 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      601 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      661 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      721 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      781 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      841 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      901 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      961 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1021 ctcaccttcg ggtgggcctt tctgcgttta ta
//