##gff-version 3
##sequence-region BBa_J54222 1 1004
BBa_J54222	.	tag	20	26	.	+	0	ID=annotation1903366;Name=BamHI
BBa_J54222	.	engineered_region	876	955	.	+	0	ID=annotation1903378;Name=BBa_B0010
BBa_J54222	.	stop_codon	997	997	.	+	0	ID=annotation1903383;Name=stop
BBa_J54222	.	stem_loop	887	930	.	+	0	ID=annotation1903379;Name=stem_loop
BBa_J54222	.	engineered_region	127	141	.	+	0	ID=annotation1903375;Name=BBa_B0030
BBa_J54222	.	ribosome_entry_site	134	138	.	+	0	ID=annotation1903376;Name=RBS
BBa_J54222	.	stem_loop	971	990	.	+	0	ID=annotation1903381;Name=T7 TE
BBa_J54222	.	engineered_region	964	1004	.	+	0	ID=annotation1903380;Name=BBa_B0012
BBa_J54222	.	polyA_site	991	1004	.	+	0	ID=annotation1903382;Name=polya
BBa_J54222	.	non_covalent_binding_site	77	92	.	+	0	ID=annotation1903369;Name=FadR
BBa_J54222	.	engineered_region	148	867	.	+	0	ID=annotation1903377;Name=BBa_E0040
BBa_J54222	.	ribosome_entry_site	127	141	.	+	0	ID=annotation1903374;Name=RBS-1\Strong
BBa_J54222	.	tag	93	99	.	+	0	ID=annotation1903370;Name=BglII
BBa_J54222	.	promoter	27	61	.	+	0	ID=annotation1903367;Name=lacq Promoter
BBa_J54222	.	engineered_region	1	5	.	+	0	ID=annotation1903365;Name=BBa_J54023
BBa_J54222	.	tag	114	118	.	+	0	ID=annotation1903372;Name=MulI
BBa_J54222	.	engineered_region	114	118	.	+	0	ID=annotation1903373;Name=BBa_J54034
BBa_J54222	.	engineered_region	14	63	.	+	0	ID=annotation1903368;Name=BBa_J54200
BBa_J54222	.	engineered_region	72	105	.	+	0	ID=annotation1903371;Name=BBa_J54220
BBa_J54222	.	tag	1	5	.	+	0	ID=annotation1903364;Name=Sal
>BBa_J54222
gtcgaccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgccgatcctgtcggagatcatctggta
cgaccagatagatctatgcatacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaactttt
cactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaa
ggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttg
tcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgc
catgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacaca
aattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaa
aattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccct
gtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatgg
tccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata