LOCUS       BBa_J54223              2232 bp    DNA     linear       25-Nov-2024
DEFINITION  ara_fadtandem
ACCESSION   BBa_J54223
VERSION     BBa_J54223.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1079
                     /label=AraC
     misc_feature    1..1210
                     /label=BBa_I0500
     promoter        1043..1114
                     /label=PC
     promoter        1080..1210
                     /label=Pbad
     misc_feature    1219..1223
                     /label=SalI
     misc_feature    1219..1237
                     /label=BBa_J54033
     misc_feature    1233..1237
                     /label=BamHI
     misc_feature    1246..1279
                     /label=BBa_J54220
     misc_feature    1251..1266
                     /label=FadR
     misc_feature    1288..1321
                     /label=BBa_J54220
     misc_feature    1293..1308
                     /label=FadR
     misc_feature    1309..1315
                     /label=BglII
     misc_feature    1330..1334
                     /label=BBa_J54034
     misc_feature    1330..1334
                     /label=MulI
     RBS             1343..1357
                     /label=RBS-1\Strong
     misc_feature    1343..1357
                     /label=BBa_B0030
     RBS             1350..1354
                     /label=RBS
     misc_feature    1364..2083
                     /label=BBa_E0040
     misc_feature    2092..2171
                     /label=BBa_B0010
     stem_loop       2103..2146
                     /label=stem_loop
     misc_feature    2180..2220
                     /label=BBa_B0012
     stem_loop       2187..2206
                     /label=T7 TE
     polyA_site      2207..2220
                     /label=polya
     misc_feature    2213..2213
                     /label=stop
     misc_feature    1..2232
                     /label=BBa_J54223
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagaggt cgaccgtgac ggatcctggt gcaaaacctt tcgcggtatg
     1261 gcatgatagc gccgatcctg tcggagatca tctggtacga ccagatagat catctggtac
     1321 gaccagatag atctatgcat acgcgttact agagattaaa gaggagaaat actagatgcg
     1381 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1441 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1501 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1561 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1621 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1681 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1741 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1801 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1861 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1921 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1981 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     2041 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     2101 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2161 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2221 tctgcgttta ta
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