LOCUS       BBa_J54224              1014 bp    DNA     linear       23-Dec-2024
DEFINITION  FadRtandem_regulated(B-21)
ACCESSION   BBa_J54224
VERSION     BBa_J54224.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    1..5
                     /label=Sal
     misc_feature    14..63
                     /label=BBa_J54200
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     misc_feature    72..105
                     /label=BBa_J54220
     misc_feature    77..92
                     /label=FadR
     misc_feature    114..147
                     /label=BBa_J54220
     misc_feature    119..134
                     /label=FadR
     misc_feature    135..141
                     /label=BglII
     misc_feature    156..160
                     /label=BBa_J54034
     misc_feature    156..160
                     /label=MulI
     RBS             169..183
                     /label=RBS-1\Strong
     misc_feature    169..183
                     /label=BBa_B0030
     RBS             176..180
                     /label=RBS
     misc_feature    190..909
                     /label=BBa_E0040
     misc_feature    918..997
                     /label=BBa_B0010
     stem_loop       929..972
                     /label=stem_loop
     misc_feature    1006..1046
                     /label=BBa_B0012
     stem_loop       1013..1032
                     /label=T7 TE
     polyA_site      1033..1046
                     /label=polya
     misc_feature    1039..1039
                     /label=stop
ORIGIN
        1 gtcgaccgtg acggatcctg gtgcaaaacc tttcgcggta tggcatgata gcgccgatcc
       61 tgtcggagat catctggtac gaccagatag atcatctggt acgaccagat agatctatgc
      121 atacgcgtta ctagagatta aagaggagaa atactagatg cgtaaaggag aagaactttt
      181 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      241 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
      301 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
      361 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
      421 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      481 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
      541 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
      601 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
      661 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
      721 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
      781 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
      841 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa
      901 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      961 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//