##gff-version 3
##sequence-region BBa_J54224 1 1046
BBa_J54224	.	engineered_region	72	105	.	+	0	ID=annotation1904534;Name=BBa_J54220
BBa_J54224	.	non_covalent_binding_site	77	92	.	+	0	ID=annotation1904535;Name=FadR
BBa_J54224	.	non_covalent_binding_site	119	134	.	+	0	ID=annotation1904537;Name=FadR
BBa_J54224	.	engineered_region	114	147	.	+	0	ID=annotation1904536;Name=BBa_J54220
BBa_J54224	.	engineered_region	169	183	.	+	0	ID=annotation1904542;Name=BBa_B0030
BBa_J54224	.	tag	156	160	.	+	0	ID=annotation1904540;Name=MulI
BBa_J54224	.	engineered_region	1006	1046	.	+	0	ID=annotation1904547;Name=BBa_B0012
BBa_J54224	.	engineered_region	14	63	.	+	0	ID=annotation1904531;Name=BBa_J54200
BBa_J54224	.	polyA_site	1033	1046	.	+	0	ID=annotation1904549;Name=polya
BBa_J54224	.	ribosome_entry_site	169	183	.	+	0	ID=annotation1904541;Name=RBS-1\Strong
BBa_J54224	.	promoter	27	61	.	+	0	ID=annotation1904533;Name=lacq Promoter
BBa_J54224	.	engineered_region	190	909	.	+	0	ID=annotation1904544;Name=BBa_E0040
BBa_J54224	.	stop_codon	1039	1039	.	+	0	ID=annotation1904550;Name=stop
BBa_J54224	.	tag	135	141	.	+	0	ID=annotation1904538;Name=BglII
BBa_J54224	.	engineered_region	156	160	.	+	0	ID=annotation1904539;Name=BBa_J54034
BBa_J54224	.	stem_loop	929	972	.	+	0	ID=annotation1904546;Name=stem_loop
BBa_J54224	.	tag	1	5	.	+	0	ID=annotation1904530;Name=Sal
BBa_J54224	.	tag	20	26	.	+	0	ID=annotation1904532;Name=BamHI
BBa_J54224	.	engineered_region	1	5	.	+	0	ID=annotation1904529;Name=BBa_J54023
BBa_J54224	.	stem_loop	1013	1032	.	+	0	ID=annotation1904548;Name=T7 TE
BBa_J54224	.	engineered_region	918	997	.	+	0	ID=annotation1904545;Name=BBa_B0010
BBa_J54224	.	ribosome_entry_site	176	180	.	+	0	ID=annotation1904543;Name=RBS
>BBa_J54224
gtcgaccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgccgatcctgtcggagatcatctggta
cgaccagatagatcatctggtacgaccagatagatctatgcatacgcgttactagagattaaagaggagaaatactaga
tgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatt
ttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaacta
cctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaa
gatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaaga
atggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaa
tactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccc
aacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactataca
aataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata