##gff-version 3
##sequence-region BBa_J54232 1 1008
BBa_J54232	.	terminator	968	1008	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J54232	.	polyA_site	995	1008	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J54232	.	stem_loop	975	994	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J54232	.	engineered_region	968	1008	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J54232	.	stop_codon	1001	1001	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J54232	.	promoter	14	63	.	+	0	ID=BBa_J54200;Name=BBa_J54200
BBa_J54232	.	tag	20	26	.	+	0	ID=annotation1903214;Name=BamHI;Parent=BBa_J54200
BBa_J54232	.	promoter	27	61	.	+	0	ID=annotation1903215;Name=lacq Promoter;Parent=BBa_J54200
BBa_J54232	.	terminator	880	959	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J54232	.	stem_loop	891	934	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J54232	.	engineered_region	880	959	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J54232	.	tag	118	122	.	+	0	ID=BBa_J54034;Name=BBa_J54034
BBa_J54232	.	tag	118	122	.	+	0	ID=annotation1903325;Name=MulI;Parent=BBa_J54034
BBa_J54232	.	promoter	72	109	.	+	0	ID=BBa_J54230;Name=BBa_J54230
BBa_J54232	.	tag	97	103	.	+	0	ID=annotation1904013;Name=BglII;Parent=BBa_J54230
BBa_J54232	.	non_covalent_binding_site	72	96	.	+	0	ID=annotation1904014;Name=TetR;Parent=BBa_J54230
BBa_J54232	.	tag	1	5	.	+	0	ID=BBa_J54023;Name=BBa_J54023
BBa_J54232	.	tag	1	5	.	+	0	ID=annotation1903320;Name=Sal;Parent=BBa_J54023
BBa_J54232	.	ribosome_entry_site	131	145	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J54232	.	ribosome_entry_site	138	142	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J54232	.	engineered_region	131	145	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J54232	.	ribosome_entry_site	131	145	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J54232	.	CDS	152	871	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J54232	.	CDS	152	871	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_J54232
gtcgatactagagccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggatcgtcc
ctatcagtgatagagatagatctatcgtaatactagagcgcgttactagagattaaagaggagaaatactagatgcgta
aaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgt
cagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgtt
ccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatg
actttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagac
acgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatgga
aacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaa
tcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcc
aattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaa
aagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata