##gff-version 3
##sequence-region BBa_J54251 1 1012
BBa_J54251	.	CDS	156	875	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J54251	.	CDS	156	875	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J54251	.	ribosome_entry_site	135	149	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J54251	.	ribosome_entry_site	142	146	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J54251	.	ribosome_entry_site	135	149	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J54251	.	engineered_region	135	149	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J54251	.	tag	1	5	.	+	0	ID=BBa_J54023;Name=BBa_J54023
BBa_J54251	.	tag	1	5	.	+	0	ID=annotation1903320;Name=Sal;Parent=BBa_J54023
BBa_J54251	.	promoter	14	63	.	+	0	ID=BBa_J54200;Name=BBa_J54200
BBa_J54251	.	tag	20	26	.	+	0	ID=annotation1903214;Name=BamHI;Parent=BBa_J54200
BBa_J54251	.	promoter	27	61	.	+	0	ID=annotation1903215;Name=lacq Promoter;Parent=BBa_J54200
BBa_J54251	.	terminator	972	1012	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J54251	.	polyA_site	999	1012	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J54251	.	engineered_region	972	1012	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J54251	.	stem_loop	979	998	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J54251	.	stop_codon	1005	1005	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J54251	.	tag	122	126	.	+	0	ID=BBa_J54034;Name=BBa_J54034
BBa_J54251	.	tag	122	126	.	+	0	ID=annotation1903325;Name=MulI;Parent=BBa_J54034
BBa_J54251	.	terminator	884	963	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J54251	.	stem_loop	895	938	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J54251	.	engineered_region	884	963	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J54251	.	promoter	72	113	.	+	0	ID=BBa_J54250;Name=BBa_J54250
BBa_J54251	.	tag	101	106	.	+	0	ID=annotation1903385;Name=BglII;Parent=BBa_J54250
BBa_J54251	.	operator	77	100	.	+	0	ID=annotation1903384;Name=LacI_Binding_Site;Parent=BBa_J54250
>BBa_J54251
gtcgatactagagccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggatcggaa
ttgtgagcggataacaattccagatctatcgtaatactagagcgcgttactagagattaaagaggagaaatactagatg
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatttt
ctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacc
tgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacag
catgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactaca
agacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaaga
tggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaat
ggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaata
ctccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaa
cgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata