LOCUS       BBa_J54252              1025 bp    DNA     linear       25-Nov-2024
DEFINITION  LacITandem_regulated(B-5-2)
ACCESSION   BBa_J54252
VERSION     BBa_J54252.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    14..63
                     /label=BBa_J54200
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     misc_feature    72..113
                     /label=BBa_J54250
     misc_feature    77..100
                     /label=LacI_Binding_Site
     misc_feature    122..163
                     /label=BBa_J54250
     misc_feature    127..150
                     /label=LacI_Binding_Site
     misc_feature    151..156
                     /label=BglII
     misc_feature    172..176
                     /label=BBa_J54034
     misc_feature    172..176
                     /label=MulI
     RBS             185..199
                     /label=RBS-1\Strong
     misc_feature    185..199
                     /label=BBa_B0030
     RBS             192..196
                     /label=RBS
     misc_feature    206..925
                     /label=BBa_E0040
     misc_feature    934..1013
                     /label=BBa_B0010
     stem_loop       945..988
                     /label=stem_loop
     misc_feature    1022..1062
                     /label=BBa_B0012
     stem_loop       1029..1048
                     /label=T7 TE
     polyA_site      1049..1062
                     /label=polya
     misc_feature    1055..1055
                     /label=stop
ORIGIN
        1 gtcgaccgtg acggatcctg gtgcaaaacc tttcgcggta tggcatgata gcgccgatcc
       61 tgtcggagat cggaattgtg agcggataac aattccagat cggaattgtg agcggataac
      121 aattccagat ctatcgtaac gcgtgagatt aaagaggaga aatactagat gcgtaaagga
      181 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      241 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      301 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      361 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      421 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      481 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      541 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      601 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      661 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      721 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
      781 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      841 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
      901 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      961 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1021 ttata
//