##gff-version 3
##sequence-region BBa_J54252 1 1062
BBa_J54252	.	ribosome_entry_site	185	199	.	+	0	ID=annotation1904135;Name=RBS-1\Strong
BBa_J54252	.	stop_codon	1055	1055	.	+	0	ID=annotation1904144;Name=stop
BBa_J54252	.	engineered_region	934	1013	.	+	0	ID=annotation1904139;Name=BBa_B0010
BBa_J54252	.	polyA_site	1049	1062	.	+	0	ID=annotation1904143;Name=polya
BBa_J54252	.	tag	172	176	.	+	0	ID=annotation1904134;Name=MulI
BBa_J54252	.	engineered_region	1	5	.	+	0	ID=annotation1904124;Name=BBa_J54023
BBa_J54252	.	tag	20	26	.	+	0	ID=annotation1904126;Name=BamHI
BBa_J54252	.	promoter	27	61	.	+	0	ID=annotation1904127;Name=lacq Promoter
BBa_J54252	.	tag	1	5	.	+	0	ID=annotation1904123;Name=Sal
BBa_J54252	.	engineered_region	1022	1062	.	+	0	ID=annotation1904141;Name=BBa_B0012
BBa_J54252	.	engineered_region	14	63	.	+	0	ID=annotation1904125;Name=BBa_J54200
BBa_J54252	.	engineered_region	185	199	.	+	0	ID=annotation1904136;Name=BBa_B0030
BBa_J54252	.	stem_loop	945	988	.	+	0	ID=annotation1904140;Name=stem_loop
BBa_J54252	.	ribosome_entry_site	192	196	.	+	0	ID=annotation1904137;Name=RBS
BBa_J54252	.	tag	151	156	.	+	0	ID=annotation1904132;Name=BglII
BBa_J54252	.	engineered_region	72	113	.	+	0	ID=annotation1904128;Name=BBa_J54250
BBa_J54252	.	engineered_region	172	176	.	+	0	ID=annotation1904133;Name=BBa_J54034
BBa_J54252	.	operator	77	100	.	+	0	ID=annotation1904129;Name=LacI_Binding_Site
BBa_J54252	.	operator	127	150	.	+	0	ID=annotation1904131;Name=LacI_Binding_Site
BBa_J54252	.	stem_loop	1029	1048	.	+	0	ID=annotation1904142;Name=T7 TE
BBa_J54252	.	engineered_region	122	163	.	+	0	ID=annotation1904130;Name=BBa_J54250
BBa_J54252	.	engineered_region	206	925	.	+	0	ID=annotation1904138;Name=BBa_E0040
>BBa_J54252
gtcgaccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgccgatcctgtcggagatcggaattgt
gagcggataacaattccagatcggaattgtgagcggataacaattccagatctatcgtaacgcgtgagattaaagagga
gaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaat
gggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcacta
ctggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccaga
tcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagat
gacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattg
attttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcaga
caaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccat
tatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcccttt
cgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgga
tgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata