LOCUS       BBa_J54254              1062 bp    DNA     linear       22-Dec-2024
DEFINITION  LacI_regulated LVA(B-5-1)
ACCESSION   BBa_J54254
VERSION     BBa_J54254.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     promoter        14..63
                     /label=BBa_J54200
     misc_feature    77..100
                     /label=LacI_Binding_Site
     misc_feature    101..106
                     /label=BglII
     promoter        72..113
                     /label=BBa_J54250
     misc_feature    127..150
                     /label=LacI_Binding_Site
     misc_feature    151..156
                     /label=BglII
     promoter        122..163
                     /label=BBa_J54250
     misc_feature    172..176
                     /label=MulI
     misc_feature    172..176
                     /label=BBa_J54034
     RBS             185..199
                     /label=RBS-1\Strong
     RBS             192..196
                     /label=RBS
     misc_feature    185..199
                     /label=BBa_B0030
     RBS             185..199
                     /label=BBa_B0030
     CDS             206..925
                     /label=GFP protein
     CDS             206..925
                     /label=BBa_E0040
     stem_loop       945..988
                     /label=stem_loop
     misc_feature    934..1013
                     /label=BBa_B0010
     terminator      934..1013
                     /label=BBa_B0010
     stem_loop       1029..1048
                     /label=T7 TE
     misc_feature    1055..1055
                     /label=stop
     polyA_site      1049..1062
                     /label=polya
     misc_feature    1022..1062
                     /label=BBa_B0012
     terminator      1022..1062
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gagccgtgac ggatcctggt gcaaaacctt tcgcggtatg gcatgatagc
       61 gcctactaga ggatcggaat tgtgagcgga taacaattcc agatctatcg taatactaga
      121 ggatcggaat tgtgagcgga taacaattcc agatctatcg taatactaga gcgcgttact
      181 agagattaaa gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt
      241 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      301 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      361 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc
      421 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
      481 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
      541 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
      601 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
      661 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
      721 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      781 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      841 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
      901 catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc
      961 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1021 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//