BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J54034
1
BBa_J54034
SuffixII_MluI
2006-10-15T11:00:00Z
2015-08-31T03:48:11Z
DNA synthesis
Restriction Enzyme site
false
false
_76_
0
762
76
Not in stock
false
As Suffix
false
Yusaku Nakashima
annotation1903325
1
MulI
range1903325
1
1
5
BBa_J54254
1
BBa_J54254
LacI_regulated LVA(B-5-1)
2006-10-23T11:00:00Z
2015-08-31T03:48:12Z
BBa_J54251
LVA TAG
false
false
_76_
0
762
76
Not in stock
true
LVA TAG
false
Yusaku Nakashima
component1904822
1
BBa_J54250
component1904828
1
BBa_E0040
component1904826
1
BBa_B0030
component1904831
1
BBa_B0012
component1904824
1
BBa_J54034
component1904829
1
BBa_B0010
component1904813
1
BBa_J54023
component1904816
1
BBa_J54200
component1904819
1
BBa_J54250
annotation1904826
1
BBa_B0030
range1904826
1
185
199
annotation1904816
1
BBa_J54200
range1904816
1
14
63
annotation1904813
1
BBa_J54023
range1904813
1
1
5
annotation1904819
1
BBa_J54250
range1904819
1
72
113
annotation1904828
1
BBa_E0040
range1904828
1
206
925
annotation1904824
1
BBa_J54034
range1904824
1
172
176
annotation1904831
1
BBa_B0012
range1904831
1
1022
1062
annotation1904822
1
BBa_J54250
range1904822
1
122
163
annotation1904829
1
BBa_B0010
range1904829
1
934
1013
BBa_J54250
1
BBa_J54250
LacI_Binding_Site
2006-10-16T11:00:00Z
2015-08-31T03:48:12Z
DNA synthesis
LacI_binding_site
false
false
_76_
0
762
76
Not in stock
false
As Regulaor
false
Yusaku Nakashima
annotation1903385
1
BglII
range1903385
1
30
35
annotation1903384
1
LacI_Binding_Site
range1903384
1
6
29
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J54200
1
BBa_J54200
lacq_Promoter
2006-10-14T11:00:00Z
2015-08-31T03:48:12Z
DNA synthesis
Promoter that express always.
false
false
_76_
0
762
76
Not in stock
false
As promoter
false
Yusaku Nakashima
annotation1903214
1
BamHI
range1903214
1
7
13
annotation1903215
1
lacq Promoter
range1903215
1
14
48
BBa_J54023
1
BBa_J54023
PrefixI_SalI
2006-10-15T11:00:00Z
2015-08-31T03:48:11Z
DNA synthesis
Resitriction Enzyme site
false
false
_76_
0
762
76
Not in stock
false
As Prefix
false
Yusaku Nakashima
annotation1903320
1
Sal
range1903320
1
1
5
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J54200_sequence
1
ccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcc
BBa_J54254_sequence
1
gtcgatactagagccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggatcggaattgtgagcggataacaattccagatctatcgtaatactagaggatcggaattgtgagcggataacaattccagatctatcgtaatactagagcgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J54034_sequence
1
cgcgt
BBa_J54250_sequence
1
gatcggaattgtgagcggataacaattccagatctatcgtaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_J54023_sequence
1
gtcga
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z