LOCUS       BBa_J54256              1108 bp    DNA     linear       09-Jun-2024
DEFINITION  AND GATE"IPTG and aTc"
ACCESSION   BBa_J54256
VERSION     BBa_J54256.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     promoter        14..63
                     /label=BBa_J54200
     misc_feature    77..100
                     /label=LacI_Binding_Site
     misc_feature    101..106
                     /label=BglII
     promoter        72..113
                     /label=BBa_J54250
     misc_feature    127..150
                     /label=LacI_Binding_Site
     misc_feature    151..156
                     /label=BglII
     promoter        122..163
                     /label=BBa_J54250
     misc_feature    197..203
                     /label=BglII
     misc_feature    172..196
                     /label=TetR
     promoter        172..209
                     /label=BBa_J54230
     misc_feature    218..222
                     /label=MulI
     misc_feature    218..222
                     /label=BBa_J54034
     RBS             231..245
                     /label=RBS-1\Strong
     RBS             238..242
                     /label=RBS
     misc_feature    231..245
                     /label=BBa_B0030
     RBS             231..245
                     /label=BBa_B0030
     CDS             252..971
                     /label=GFP protein
     CDS             252..971
                     /label=BBa_E0040
     stem_loop       991..1034
                     /label=stem_loop
     misc_feature    980..1059
                     /label=BBa_B0010
     terminator      980..1059
                     /label=BBa_B0010
     stem_loop       1075..1094
                     /label=T7 TE
     misc_feature    1101..1101
                     /label=stop
     polyA_site      1095..1108
                     /label=polya
     misc_feature    1068..1108
                     /label=BBa_B0012
     terminator      1068..1108
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gagccgtgac ggatcctggt gcaaaacctt tcgcggtatg gcatgatagc
       61 gcctactaga ggatcggaat tgtgagcgga taacaattcc agatctatcg taatactaga
      121 ggatcggaat tgtgagcgga taacaattcc agatctatcg taatactaga ggatcgtccc
      181 tatcagtgat agagatagat ctatcgtaat actagagcgc gttactagag attaaagagg
      241 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      301 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      361 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      421 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      481 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      541 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      601 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      661 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      721 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      781 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      841 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
      901 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
      961 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1021 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1081 ccttcgggtg ggcctttctg cgtttata
//