##gff-version 3
##sequence-region BBa_J54256 1 1108
BBa_J54256	.	tag	218	222	.	+	0	ID=BBa_J54034;Name=BBa_J54034
BBa_J54256	.	tag	218	222	.	+	0	ID=annotation1903325;Name=MulI;Parent=BBa_J54034
BBa_J54256	.	terminator	980	1059	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J54256	.	stem_loop	991	1034	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J54256	.	engineered_region	980	1059	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J54256	.	promoter	14	63	.	+	0	ID=BBa_J54200;Name=BBa_J54200
BBa_J54256	.	promoter	27	61	.	+	0	ID=annotation1903215;Name=lacq Promoter;Parent=BBa_J54200
BBa_J54256	.	tag	20	26	.	+	0	ID=annotation1903214;Name=BamHI;Parent=BBa_J54200
BBa_J54256	.	tag	1	5	.	+	0	ID=BBa_J54023;Name=BBa_J54023
BBa_J54256	.	tag	1	5	.	+	0	ID=annotation1903320;Name=Sal;Parent=BBa_J54023
BBa_J54256	.	promoter	172	209	.	+	0	ID=BBa_J54230;Name=BBa_J54230
BBa_J54256	.	tag	197	203	.	+	0	ID=annotation1904013;Name=BglII;Parent=BBa_J54230
BBa_J54256	.	non_covalent_binding_site	172	196	.	+	0	ID=annotation1904014;Name=TetR;Parent=BBa_J54230
BBa_J54256	.	promoter	122	163	.	+	0	ID=BBa_J54250;Name=BBa_J54250
BBa_J54256	.	operator	127	150	.	+	0	ID=annotation1903384;Name=LacI_Binding_Site;Parent=BBa_J54250
BBa_J54256	.	tag	151	156	.	+	0	ID=annotation1903385;Name=BglII;Parent=BBa_J54250
BBa_J54256	.	promoter	72	113	.	+	0	ID=BBa_J54250;Name=BBa_J54250
BBa_J54256	.	operator	77	100	.	+	0	ID=annotation1903384;Name=LacI_Binding_Site;Parent=BBa_J54250
BBa_J54256	.	tag	101	106	.	+	0	ID=annotation1903385;Name=BglII;Parent=BBa_J54250
BBa_J54256	.	CDS	252	971	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J54256	.	CDS	252	971	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J54256	.	terminator	1068	1108	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J54256	.	stem_loop	1075	1094	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J54256	.	stop_codon	1101	1101	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J54256	.	polyA_site	1095	1108	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J54256	.	engineered_region	1068	1108	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J54256	.	ribosome_entry_site	231	245	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J54256	.	ribosome_entry_site	238	242	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J54256	.	engineered_region	231	245	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J54256	.	ribosome_entry_site	231	245	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J54256
gtcgatactagagccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggatcggaa
ttgtgagcggataacaattccagatctatcgtaatactagaggatcggaattgtgagcggataacaattccagatctat
cgtaatactagaggatcgtccctatcagtgatagagatagatctatcgtaatactagagcgcgttactagagattaaag
aggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgt
taatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgc
actactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacc
cagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaa
agatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggt
attgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatgg
cagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcaga
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcc
ctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggca
tggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta