BBa_J54200
1
BBa_J54200
lacq_Promoter
2006-10-14T11:00:00Z
2015-08-31T03:48:12Z
DNA synthesis
Promoter that express always.
false
false
_76_
0
762
76
Not in stock
false
As promoter
false
Yusaku Nakashima
annotation1903215
1
lacq Promoter
range1903215
1
14
48
annotation1903214
1
BamHI
range1903214
1
7
13
BBa_J54256
1
BBa_J54256
AND GATE"IPTG and aTc"
2006-10-23T11:00:00Z
2015-08-31T03:48:12Z
BBa_J54252
This promoter is regulated by IPTG and aTc.
false
false
_76_
0
762
76
Not in stock
false
AND GATE
false
Yusaku Nakashima
component1904878
1
BBa_B0030
component1904868
1
BBa_J54250
component1904865
1
BBa_J54200
component1904876
1
BBa_J54034
component1904881
1
BBa_B0010
component1904880
1
BBa_E0040
component1904862
1
BBa_J54023
component1904874
1
BBa_J54230
component1904871
1
BBa_J54250
component1904883
1
BBa_B0012
annotation1904881
1
BBa_B0010
range1904881
1
980
1059
annotation1904868
1
BBa_J54250
range1904868
1
72
113
annotation1904880
1
BBa_E0040
range1904880
1
252
971
annotation1904883
1
BBa_B0012
range1904883
1
1068
1108
annotation1904871
1
BBa_J54250
range1904871
1
122
163
annotation1904876
1
BBa_J54034
range1904876
1
218
222
annotation1904865
1
BBa_J54200
range1904865
1
14
63
annotation1904878
1
BBa_B0030
range1904878
1
231
245
annotation1904862
1
BBa_J54023
range1904862
1
1
5
annotation1904874
1
BBa_J54230
range1904874
1
172
209
BBa_J54034
1
BBa_J54034
SuffixII_MluI
2006-10-15T11:00:00Z
2015-08-31T03:48:11Z
DNA synthesis
Restriction Enzyme site
false
false
_76_
0
762
76
Not in stock
false
As Suffix
false
Yusaku Nakashima
annotation1903325
1
MulI
range1903325
1
1
5
BBa_J54230
1
BBa_J54230
TetR_regulated
2006-10-21T11:00:00Z
2015-08-31T03:48:12Z
DNA synthesis
TetR_binding_site
false
false
_76_
0
762
76
Not in stock
false
a
false
Yusaku Nakashima
annotation1904013
1
BglII
range1904013
1
26
32
annotation1904014
1
TetR
range1904014
1
1
25
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_J54250
1
BBa_J54250
LacI_Binding_Site
2006-10-16T11:00:00Z
2015-08-31T03:48:12Z
DNA synthesis
LacI_binding_site
false
false
_76_
0
762
76
Not in stock
false
As Regulaor
false
Yusaku Nakashima
annotation1903384
1
LacI_Binding_Site
range1903384
1
6
29
annotation1903385
1
BglII
range1903385
1
30
35
BBa_J54023
1
BBa_J54023
PrefixI_SalI
2006-10-15T11:00:00Z
2015-08-31T03:48:11Z
DNA synthesis
Resitriction Enzyme site
false
false
_76_
0
762
76
Not in stock
false
As Prefix
false
Yusaku Nakashima
annotation1903320
1
Sal
range1903320
1
1
5
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J54230_sequence
1
gatcgtccctatcagtgatagagatagatctatcgtaa
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J54200_sequence
1
ccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcc
BBa_J54034_sequence
1
cgcgt
BBa_J54250_sequence
1
gatcggaattgtgagcggataacaattccagatctatcgtaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J54256_sequence
1
gtcgatactagagccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggatcggaattgtgagcggataacaattccagatctatcgtaatactagaggatcggaattgtgagcggataacaattccagatctatcgtaatactagaggatcgtccctatcagtgatagagatagatctatcgtaatactagagcgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_J54023_sequence
1
gtcga
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z