LOCUS       BBa_J54401               918 bp    DNA     linear       29-Nov-2024
DEFINITION  TyrS_ptomoter(D-1)
ACCESSION   BBa_J54401
VERSION     BBa_J54401.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    14..18
                     /label=BglII
     misc_feature    27..32
                     /label=MulI
     misc_feature    14..32
                     /label=BBa_J54025
     RBS             41..55
                     /label=RBS-1\Strong
     RBS             48..52
                     /label=RBS
     misc_feature    41..55
                     /label=BBa_B0030
     RBS             41..55
                     /label=BBa_B0030
     CDS             62..781
                     /label=GFP protein
     CDS             62..781
                     /label=BBa_E0040
     stem_loop       801..844
                     /label=stem_loop
     misc_feature    790..869
                     /label=BBa_B0010
     terminator      790..869
                     /label=BBa_B0010
     stem_loop       885..904
                     /label=T7 TE
     misc_feature    911..911
                     /label=stop
     polyA_site      905..918
                     /label=polya
     misc_feature    878..918
                     /label=BBa_B0012
     terminator      878..918
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gaggatctgt cggataacgc gttactagag attaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttata
//