##gff-version 3
##sequence-region BBa_J55000 1 1926
BBa_J55000	.	promoter	988	1042	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J55000	.	start_codon	1040	1040	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J55000	.	promoter	1029	1034	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J55000	.	promoter	1007	1012	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J55000	.	engineered_region	988	1042	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J55000	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J55000	.	engineered_region	1051	1926	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_J55000	.	terminator	1886	1926	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_J55000	.	stop_codon	1919	1919	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J55000	.	polyA_site	1913	1926	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J55000	.	stem_loop	1893	1912	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J55000	.	engineered_region	1886	1926	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J55000	.	terminator	1798	1877	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_J55000	.	engineered_region	1798	1877	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J55000	.	stem_loop	1809	1852	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J55000	.	ribosome_entry_site	1051	1063	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_J55000	.	ribosome_entry_site	1051	1063	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J55000	.	engineered_region	1051	1063	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J55000	.	ribosome_entry_site	1057	1060	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J55000	.	CDS	1070	1789	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_J55000	.	CDS	1070	1789	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J55000	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_J55000	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_J55000	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J55000	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J55000	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J55000	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J55000	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_J55000	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J55000	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J55000	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J55000	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J55000	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J55000	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J55000	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_J55000	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J55000	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J55000	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_J55000	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J55000	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_J55000
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaataaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcc
caattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacata
cggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggt
tatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtt
atgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatac
ccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaac
tataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaaca
ttgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccaga
caaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagttt
gtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata