LOCUS BBa_J5517 3473 bp DNA linear 22-Nov-2024 DEFINITION Arabinose -> LacI ts -| GFP ACCESSION BBa_J5517 VERSION BBa_J5517.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2364 /label=LacI(ts)-LVA misc_feature 2039..2039 /label=TS mutation (G->A) misc_feature 2326..2364 /label=LVA misc_feature 2365..2389 /label=barcode from LacI (BBa_C0012) CDS 1237..2389 /label=BBa_J06501 stem_loop 2409..2452 /label=stem_loop misc_feature 2398..2477 /label=BBa_B0010 terminator 2398..2477 /label=BBa_B0010 stem_loop 2493..2512 /label=T7 TE misc_feature 2519..2519 /label=stop polyA_site 2513..2526 /label=polya misc_feature 2486..2526 /label=BBa_B0012 terminator 2486..2526 /label=BBa_B0012 misc_feature 2537..2553 /label=lac O1 promoter 2554..2559 /label=-35 misc_feature 2560..2576 /label=lac O1 promoter 2577..2582 /label=-10 misc_feature 2535..2588 /label=BBa_R0011 promoter 2535..2588 /label=BBa_R0011 RBS 2598..2610 /label=RBS-3\Weak RBS 2604..2607 /label=RBS misc_feature 2598..2610 /label=BBa_B0032 RBS 2598..2610 /label=BBa_B0032 CDS 2617..3336 /label=GFP protein CDS 2617..3336 /label=BBa_E0040 stem_loop 3356..3399 /label=stem_loop misc_feature 3345..3424 /label=BBa_B0010 terminator 3345..3424 /label=BBa_B0010 stem_loop 3440..3459 /label=T7 TE misc_feature 3466..3466 /label=stop polyA_site 3460..3473 /label=polya misc_feature 3433..3473 /label=BBa_B0012 terminator 3433..3473 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttgat 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2521 tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata 2581 ctgagcacat actagagtca cacaggaaag tactagatgc gtaaaggaga agaacttttc 2641 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 2701 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 2761 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt 2821 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 2881 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 2941 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 3001 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 3061 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 3121 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 3181 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 3241 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 3301 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa 3361 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 3421 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //