##gff-version 3
##sequence-region BBa_J5521 1 3499
BBa_J5521	.	engineered_region	1219	2589	.	+	0	ID=BBa_J06801;Name=BBa_J06801
BBa_J5521	.	CDS	1237	2389	.	+	0	ID=BBa_J06501;Name=BBa_J06501;Parent=BBa_J06801
BBa_J5521	.	sequence_alteration	2039	2039	.	+	0	ID=annotation1530900;Name=TS mutation (G->A);Parent=BBa_J06501
BBa_J5521	.	tag	2326	2364	.	+	0	ID=annotation1530907;Name=LVA;Parent=BBa_J06501
BBa_J5521	.	engineered_tag	2365	2389	.	+	0	ID=annotation1530914;Name=barcode from LacI (BBa_C0012);Parent=BBa_J06501
BBa_J5521	.	CDS	1237	2364	.	+	0	ID=annotation1530899;Name=LacI(ts)-LVA;Parent=BBa_J06501
BBa_J5521	.	terminator	2486	2526	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06801
BBa_J5521	.	engineered_region	2486	2526	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J5521	.	stop_codon	2519	2519	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J5521	.	stem_loop	2493	2512	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J5521	.	polyA_site	2513	2526	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J5521	.	promoter	2535	2588	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J06801
BBa_J5521	.	promoter	2554	2559	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J5521	.	promoter	2577	2582	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J5521	.	operator	2537	2553	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J5521	.	operator	2560	2576	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J5521	.	engineered_region	2535	2588	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J5521	.	terminator	2398	2477	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06801
BBa_J5521	.	stem_loop	2409	2452	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J5521	.	engineered_region	2398	2477	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J5521	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06801
BBa_J5521	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J5521	.	engineered_region	2598	3499	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_J5521	.	ribosome_entry_site	2598	2609	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_J5521	.	conserved_region	2602	2605	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J5521	.	CDS	2616	3275	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_J5521	.	engineered_tag	3276	3300	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J5521	.	tag	3236	3269	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J5521	.	CDS	2619	3235	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J5521	.	terminator	3397	3437	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_J5521	.	engineered_region	3397	3437	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J5521	.	stop_codon	3430	3430	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J5521	.	stem_loop	3404	3423	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J5521	.	polyA_site	3424	3437	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J5521	.	promoter	3446	3499	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_J5521	.	promoter	3465	3470	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J5521	.	non_covalent_binding_site	3471	3489	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J5521	.	engineered_region	3446	3499	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J5521	.	non_covalent_binding_site	3446	3464	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J5521	.	promoter	3488	3493	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J5521	.	terminator	3309	3388	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_J5521	.	stem_loop	3320	3363	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J5521	.	engineered_region	3309	3388	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J5521	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_J5521	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J5521	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J5521	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
>BBa_J5521
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttat
acgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaa
aacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaa
cagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctc
gcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgca
caatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagct
gcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaag
acggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttc
tgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaa
ggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctgg
ttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttgatgcggatatctcggt
agtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctg
gggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac
tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct
ggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctga
tagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggaga
aatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaagg
tttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggct
ttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaag
attttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtac
acggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaat
gcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaag
aagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgc
agagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgct
gcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatcccta
tcagtgatagagatactgagcac