##gff-version 3
##sequence-region BBa_J5522 1 3498
BBa_J5522	.	engineered_region	1219	2589	.	+	0	ID=BBa_J06801;Name=BBa_J06801
BBa_J5522	.	CDS	1237	2389	.	+	0	ID=BBa_J06501;Name=BBa_J06501;Parent=BBa_J06801
BBa_J5522	.	sequence_alteration	2039	2039	.	+	0	ID=annotation1530900;Name=TS mutation (G->A);Parent=BBa_J06501
BBa_J5522	.	tag	2326	2364	.	+	0	ID=annotation1530907;Name=LVA;Parent=BBa_J06501
BBa_J5522	.	engineered_tag	2365	2389	.	+	0	ID=annotation1530914;Name=barcode from LacI (BBa_C0012);Parent=BBa_J06501
BBa_J5522	.	CDS	1237	2364	.	+	0	ID=annotation1530899;Name=LacI(ts)-LVA;Parent=BBa_J06501
BBa_J5522	.	terminator	2486	2526	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06801
BBa_J5522	.	engineered_region	2486	2526	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J5522	.	stop_codon	2519	2519	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J5522	.	stem_loop	2493	2512	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J5522	.	polyA_site	2513	2526	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J5522	.	promoter	2535	2588	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J06801
BBa_J5522	.	promoter	2554	2559	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J5522	.	promoter	2577	2582	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J5522	.	operator	2537	2553	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J5522	.	operator	2560	2576	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J5522	.	engineered_region	2535	2588	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J5522	.	terminator	2398	2477	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06801
BBa_J5522	.	stem_loop	2409	2452	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J5522	.	engineered_region	2398	2477	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J5522	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06801
BBa_J5522	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J5522	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_J5522	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J5522	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J5522	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J5522	.	engineered_region	2598	3498	.	+	0	ID=BBa_Q03400;Name=BBa_Q03400
BBa_J5522	.	ribosome_entry_site	2598	2608	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_Q03400
BBa_J5522	.	ribosome_entry_site	2604	2607	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J5522	.	ribosome_entry_site	2598	2608	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J5522	.	engineered_region	2598	2608	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J5522	.	terminator	3396	3436	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q03400
BBa_J5522	.	engineered_region	3396	3436	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J5522	.	stop_codon	3429	3429	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J5522	.	stem_loop	3403	3422	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J5522	.	polyA_site	3423	3436	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J5522	.	CDS	2615	3274	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q03400
BBa_J5522	.	engineered_tag	3275	3299	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J5522	.	tag	3235	3268	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J5522	.	CDS	2618	3234	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J5522	.	promoter	3445	3498	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q03400
BBa_J5522	.	promoter	3464	3469	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J5522	.	non_covalent_binding_site	3470	3488	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J5522	.	engineered_region	3445	3498	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J5522	.	non_covalent_binding_site	3445	3463	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J5522	.	promoter	3487	3492	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J5522	.	terminator	3308	3387	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q03400
BBa_J5522	.	stem_loop	3319	3362	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J5522	.	engineered_region	3308	3387	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J5522
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttat
acgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaa
aacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaa
cagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctc
gcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgca
caatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagct
gcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaag
acggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttc
tgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaa
ggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctgg
ttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttgatgcggatatctcggt
agtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctg
gggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac
tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct
ggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctga
tagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacagga
ctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggt
ttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctt
tgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaaga
ttttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtaca
cggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatg
cattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaaga
agaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgca
gagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctg
caaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctat
cagtgatagagatactgagcac