LOCUS BBa_I13520 2079 bp DNA linear 22-Dec-2024 DEFINITION Pbad mRFP ACCESSION BBa_I13520 VERSION BBa_I13520.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1911 /label=mrfp1 misc_feature 1918..1942 /label=Help:Barcodes CDS 1237..1917 /label=BBa_E1010 stem_loop 1962..2005 /label=stem_loop misc_feature 1951..2030 /label=BBa_B0010 terminator 1951..2030 /label=BBa_B0010 stem_loop 2046..2065 /label=T7 TE misc_feature 2072..2072 /label=stop polyA_site 2066..2079 /label=polya misc_feature 2039..2079 /label=BBa_B0012 terminator 2039..2079 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc 1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc 1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa 1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag 1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac 1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc 1921 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 1981 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 2041 acactggctc accttcgggt gggcctttct gcgtttata // LOCUS BBa_J5523 2079 bp DNA linear 22-Dec-2024 DEFINITION LacI -| mRFP ACCESSION BBa_J5523 VERSION BBa_J5523.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1911 /label=mrfp1 misc_feature 1918..1942 /label=Help:Barcodes CDS 1237..1917 /label=BBa_E1010 stem_loop 1962..2005 /label=stem_loop misc_feature 1951..2030 /label=BBa_B0010 terminator 1951..2030 /label=BBa_B0010 stem_loop 2046..2065 /label=T7 TE misc_feature 2072..2072 /label=stop polyA_site 2066..2079 /label=polya misc_feature 2039..2079 /label=BBa_B0012 terminator 2039..2079 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc 1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc 1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa 1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag 1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac 1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc 1921 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 1981 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 2041 acactggctc accttcgggt gggcctttct gcgtttata //