LOCUS BBa_J5525 4381 bp DNA linear 23-May-2024 DEFINITION Arabinose -> LacI -| tetR -| GFP ACCESSION BBa_J5525 VERSION BBa_J5525.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2364 /label=LacI(ts)-LVA misc_feature 2039..2039 /label=TS mutation (G->A) misc_feature 2326..2364 /label=LVA misc_feature 2365..2389 /label=barcode from LacI (BBa_C0012) CDS 1237..2389 /label=BBa_J06501 stem_loop 2409..2452 /label=stem_loop misc_feature 2398..2477 /label=BBa_B0010 terminator 2398..2477 /label=BBa_B0010 stem_loop 2493..2512 /label=T7 TE misc_feature 2519..2519 /label=stop polyA_site 2513..2526 /label=polya misc_feature 2486..2526 /label=BBa_B0012 terminator 2486..2526 /label=BBa_B0012 misc_feature 2537..2553 /label=lac O1 promoter 2554..2559 /label=-35 misc_feature 2560..2576 /label=lac O1 promoter 2577..2582 /label=-10 misc_feature 2535..2588 /label=BBa_R0011 promoter 2535..2588 /label=BBa_R0011 misc_feature 1219..2589 /label=BBa_J06801 RBS 2598..2608 /label=RBS-4\Weaker RBS 2604..2607 /label=RBS misc_feature 2598..2608 /label=BBa_B0033 RBS 2598..2608 /label=BBa_B0033 CDS 2618..3234 /label=tetR misc_feature 3235..3268 /label=SsrA misc_feature 3275..3299 /label=Help:Barcodes CDS 2615..3274 /label=BBa_C0040 stem_loop 3319..3362 /label=stem_loop misc_feature 3308..3387 /label=BBa_B0010 terminator 3308..3387 /label=BBa_B0010 stem_loop 3403..3422 /label=T7 TE misc_feature 3429..3429 /label=stop polyA_site 3423..3436 /label=polya misc_feature 3396..3436 /label=BBa_B0012 terminator 3396..3436 /label=BBa_B0012 misc_feature 3445..3463 /label=TetR 1 misc_feature 3445..3498 /label=BBa_R0040 promoter 3464..3469 /label=-35 misc_feature 3470..3488 /label=TetR 2 promoter 3487..3492 /label=-10 promoter 3445..3498 /label=BBa_R0040 misc_feature 2598..3498 /label=BBa_Q03400 misc_feature 3511..3514 /label=conserved RBS 3507..3518 /label=BBa_B0034 CDS 3525..4244 /label=GFP protein CDS 3525..4244 /label=BBa_E0040 stem_loop 4264..4307 /label=stem_loop misc_feature 4253..4332 /label=BBa_B0010 terminator 4253..4332 /label=BBa_B0010 stem_loop 4348..4367 /label=T7 TE misc_feature 4374..4374 /label=stop polyA_site 4368..4381 /label=polya misc_feature 4341..4381 /label=BBa_B0012 terminator 4341..4381 /label=BBa_B0012 misc_feature 3507..4381 /label=BBa_I13504 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttgat 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2521 tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata 2581 ctgagcacat actagagtca cacaggacta ctagatgtcc agattagata aaagtaaagt 2641 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa 2701 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg 2761 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc 2821 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc 2881 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca 2941 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa 3001 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca 3061 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt 3121 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg 3181 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc 3241 aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact 3301 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 3361 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 3421 cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt 3481 gatagagata ctgagcacta ctagagaaag aggagaaata ctagatgcgt aaaggagaag 3541 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca 3601 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat 3661 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt 3721 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga 3781 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact 3841 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa 3901 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata 3961 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca 4021 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata 4081 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg 4141 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 4201 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat 4261 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4321 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4381 a //