LOCUS BBa_J5530 5314 bp DNA linear 27-Dec-2024 DEFINITION Cell-See-Us Bacterial Thermometer ACCESSION BBa_J5530 VERSION BBa_J5530.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2364 /label=LacI(ts)-LVA misc_feature 2039..2039 /label=TS mutation (G->A) misc_feature 2326..2364 /label=LVA misc_feature 2365..2389 /label=barcode from LacI (BBa_C0012) CDS 1237..2389 /label=BBa_J06501 stem_loop 2409..2452 /label=stem_loop misc_feature 2398..2477 /label=BBa_B0010 terminator 2398..2477 /label=BBa_B0010 stem_loop 2493..2512 /label=T7 TE misc_feature 2519..2519 /label=stop polyA_site 2513..2526 /label=polya misc_feature 2486..2526 /label=BBa_B0012 terminator 2486..2526 /label=BBa_B0012 misc_feature 2537..2553 /label=lac O1 promoter 2554..2559 /label=-35 misc_feature 2560..2576 /label=lac O1 promoter 2577..2582 /label=-10 misc_feature 2535..2588 /label=BBa_R0011 promoter 2535..2588 /label=BBa_R0011 misc_feature 1219..2589 /label=BBa_J06801 misc_feature 2602..2605 /label=conserved RBS 2598..2609 /label=BBa_B0034 CDS 2619..3235 /label=tetR misc_feature 3236..3269 /label=SsrA misc_feature 3276..3300 /label=Help:Barcodes CDS 2616..3275 /label=BBa_C0040 stem_loop 3320..3363 /label=stem_loop misc_feature 3309..3388 /label=BBa_B0010 terminator 3309..3388 /label=BBa_B0010 stem_loop 3404..3423 /label=T7 TE misc_feature 3430..3430 /label=stop polyA_site 3424..3437 /label=polya misc_feature 3397..3437 /label=BBa_B0012 terminator 3397..3437 /label=BBa_B0012 misc_feature 3446..3464 /label=TetR 1 misc_feature 3446..3499 /label=BBa_R0040 promoter 3465..3470 /label=-35 misc_feature 3471..3489 /label=TetR 2 promoter 3488..3493 /label=-10 promoter 3446..3499 /label=BBa_R0040 misc_feature 2598..3499 /label=BBa_Q04400 misc_feature 3512..3515 /label=conserved RBS 3508..3519 /label=BBa_B0034 CDS 3526..4245 /label=GFP protein CDS 3526..4245 /label=BBa_E0040 stem_loop 4265..4308 /label=stem_loop misc_feature 4254..4333 /label=BBa_B0010 terminator 4254..4333 /label=BBa_B0010 stem_loop 4349..4368 /label=T7 TE misc_feature 4375..4375 /label=stop polyA_site 4369..4382 /label=polya misc_feature 4342..4382 /label=BBa_B0012 terminator 4342..4382 /label=BBa_B0012 misc_feature 3508..4382 /label=BBa_I13504 misc_feature 4393..4409 /label=lac O1 promoter 4410..4415 /label=-35 misc_feature 4416..4432 /label=lac O1 promoter 4433..4438 /label=-10 misc_feature 4391..4444 /label=BBa_R0011 promoter 4391..4444 /label=BBa_R0011 misc_feature 4458..4461 /label=conserved RBS 4454..4465 /label=BBa_B0034 CDS 4472..5146 /label=mrfp1 misc_feature 5153..5177 /label=Help:Barcodes CDS 4472..5152 /label=BBa_E1010 stem_loop 5197..5240 /label=stem_loop misc_feature 5186..5265 /label=BBa_B0010 terminator 5186..5265 /label=BBa_B0010 stem_loop 5281..5300 /label=T7 TE misc_feature 5307..5307 /label=stop polyA_site 5301..5314 /label=polya misc_feature 5274..5314 /label=BBa_B0012 terminator 5274..5314 /label=BBa_B0012 misc_feature 4454..5314 /label=BBa_I13507 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttgat 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2521 tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata 2581 ctgagcacat actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag 2641 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 2701 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 2761 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 2821 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 2881 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 2941 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 3001 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 3061 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 3121 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 3181 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg 3241 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 3301 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 3361 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3421 gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag 3481 tgatagagat actgagcact actagagaaa gaggagaaat actagatgcg taaaggagaa 3541 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac 3601 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa 3661 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt 3721 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag 3781 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac 3841 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta 3901 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat 3961 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc 4021 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat 4081 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct 4141 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca 4201 gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca 4261 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 4321 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 4381 tatactagag aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga 4441 gcacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 4501 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 4561 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 4621 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 4681 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 4741 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 4801 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 4861 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 4921 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 4981 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 5041 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 5101 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 5161 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 5221 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 5281 ggctcacctt cgggtgggcc tttctgcgtt tata //