##gff-version 3
##sequence-region BBa_J5531 1 965
BBa_J5531	.	engineered_region	64	965	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_J5531	.	terminator	775	854	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_J5531	.	stem_loop	786	829	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J5531	.	engineered_region	775	854	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J5531	.	CDS	82	741	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_J5531	.	tag	702	735	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J5531	.	engineered_tag	742	766	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J5531	.	CDS	85	701	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J5531	.	promoter	912	965	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_J5531	.	engineered_region	912	965	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J5531	.	promoter	931	936	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J5531	.	non_covalent_binding_site	912	930	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J5531	.	promoter	954	959	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J5531	.	non_covalent_binding_site	937	955	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J5531	.	terminator	863	903	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_J5531	.	stem_loop	870	889	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J5531	.	stop_codon	896	896	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J5531	.	polyA_site	890	903	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J5531	.	engineered_region	863	903	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J5531	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_J5531	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J5531	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J5531	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J5531	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J5531	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J5531	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J5531	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
>BBa_J5531
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcac