LOCUS       BBa_J5532               1750 bp    DNA     linear       23-Dec-2024
DEFINITION  inv
ACCESSION   BBa_J5532
VERSION     BBa_J5532.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    1..902
                     /label=BBa_Q04400
     misc_feature    915..918
                     /label=conserved
     RBS             911..922
                     /label=BBa_B0034
     CDS             932..1548
                     /label=tetR
     misc_feature    1549..1582
                     /label=SsrA
     misc_feature    1589..1613
                     /label=Help:Barcodes
     CDS             929..1588
                     /label=BBa_C0040
     stem_loop       1633..1676
                     /label=stem_loop
     misc_feature    1622..1701
                     /label=BBa_B0010
     terminator      1622..1701
                     /label=BBa_B0010
     stem_loop       1717..1736
                     /label=T7 TE
     misc_feature    1743..1743
                     /label=stop
     polyA_site      1737..1750
                     /label=polya
     misc_feature    1710..1750
                     /label=BBa_B0012
     terminator      1710..1750
                     /label=BBa_B0012
     misc_feature    911..1750
                     /label=BBa_P0440
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa
      961 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     1021 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     1081 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
     1141 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
     1201 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
     1261 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
     1321 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
     1381 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
     1441 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
     1501 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga
     1561 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga
     1621 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1681 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1741 tgcgtttata
//