##gff-version 3
##sequence-region BBa_J5532 1 1750
BBa_J5532	.	engineered_region	911	1750	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_J5532	.	terminator	1622	1701	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_J5532	.	stem_loop	1633	1676	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J5532	.	engineered_region	1622	1701	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J5532	.	CDS	929	1588	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_J5532	.	CDS	932	1548	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J5532	.	tag	1549	1582	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J5532	.	engineered_tag	1589	1613	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J5532	.	ribosome_entry_site	911	922	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_J5532	.	conserved_region	915	918	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J5532	.	terminator	1710	1750	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_J5532	.	stem_loop	1717	1736	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J5532	.	stop_codon	1743	1743	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J5532	.	polyA_site	1737	1750	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J5532	.	engineered_region	1710	1750	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J5532	.	engineered_region	1	902	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_J5532	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_J5532	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J5532	.	terminator	712	791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_J5532	.	stem_loop	723	766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J5532	.	engineered_region	712	791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J5532	.	promoter	849	902	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_J5532	.	promoter	868	873	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J5532	.	engineered_region	849	902	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J5532	.	non_covalent_binding_site	849	867	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J5532	.	promoter	891	896	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J5532	.	non_covalent_binding_site	874	892	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J5532	.	terminator	800	840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_J5532	.	stem_loop	807	826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J5532	.	stop_codon	833	833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J5532	.	polyA_site	827	840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J5532	.	engineered_region	800	840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J5532	.	CDS	19	678	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_J5532	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J5532	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J5532	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_J5532
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtccagattagataaaag
taaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaag
ctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgt
tagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttt
tagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaact
ctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggc
attttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatag
tatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaa
ttgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtag
cttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata