LOCUS       BBa_J58010               920 bp    DNA     linear       25-Dec-2024
DEFINITION  A gene to characterize GFP using IPTG
ACCESSION   BBa_J58010
VERSION     BBa_J58010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     CDS             64..783
                     /label=GFP protein
     CDS             64..783
                     /label=BBa_E0040
     stem_loop       803..846
                     /label=stem_loop
     misc_feature    792..871
                     /label=BBa_B0010
     terminator      792..871
                     /label=BBa_B0010
     stem_loop       887..906
                     /label=T7 TE
     misc_feature    913..913
                     /label=stop
     polyA_site      907..920
                     /label=polya
     misc_feature    880..920
                     /label=BBa_B0012
     terminator      880..920
                     /label=BBa_B0012
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      121 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      181 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      241 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      301 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      361 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      421 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      481 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      541 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      601 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      661 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      721 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
      781 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      901 tgggcctttc tgcgtttata
//