LOCUS       BBa_J58012               872 bp    DNA     linear       09-May-2024
DEFINITION  Promoter activated synergistically by cI and CRP with the reporter 
            RFP
ACCESSION   BBa_J58012
VERSION     BBa_J58012.1  
FEATURES             Location/Qualifiers
     promoter        1..3
                     /label=BBa_J58011
     misc_feature    16..19
                     /label=conserved
     RBS             12..23
                     /label=BBa_B0034
     CDS             30..704
                     /label=mrfp1
     misc_feature    711..735
                     /label=Help:Barcodes
     CDS             30..710
                     /label=BBa_E1010
     stem_loop       755..798
                     /label=stem_loop
     misc_feature    744..823
                     /label=BBa_B0010
     terminator      744..823
                     /label=BBa_B0010
     stem_loop       839..858
                     /label=T7 TE
     misc_feature    865..865
                     /label=stop
     polyA_site      859..872
                     /label=polya
     misc_feature    832..872
                     /label=BBa_B0012
     terminator      832..872
                     /label=BBa_B0012
ORIGIN
        1 aaatactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
       61 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
      121 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
      181 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
      241 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
      301 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
      361 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
      421 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
      481 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
      541 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
      601 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
      661 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt
      721 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      781 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      841 ctcaccttcg ggtgggcctt tctgcgttta ta
//