LOCUS       BBa_J58014               991 bp    DNA     linear       23-Dec-2024
DEFINITION  Promoter activated by OmpR-P with the reporter GFP
ACCESSION   BBa_J58014
VERSION     BBa_J58014.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             135..854
                     /label=GFP protein
     CDS             135..854
                     /label=BBa_E0040
     stem_loop       874..917
                     /label=stem_loop
     misc_feature    863..942
                     /label=BBa_B0010
     terminator      863..942
                     /label=BBa_B0010
     stem_loop       958..977
                     /label=T7 TE
     misc_feature    984..984
                     /label=stop
     polyA_site      978..991
                     /label=polya
     misc_feature    951..991
                     /label=BBa_B0012
     terminator      951..991
                     /label=BBa_B0012
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      181 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      241 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      301 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
      361 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      421 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      481 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      541 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      601 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      661 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      721 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
      781 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
      841 tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      901 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      961 tcaccttcgg gtgggccttt ctgcgtttat a
//