LOCUS       BBa_J58101               975 bp    DNA     linear       08-May-2024
DEFINITION  Promoter activated synergistically by cI and CRP with the reporter 
            RFP
ACCESSION   BBa_J58101
VERSION     BBa_J58101.1  
FEATURES             Location/Qualifiers
     misc_feature    3..24
                     /label=CRP binding site
     misc_feature    57..74
                     /label=lam-cI OR2 binding site
     misc_feature    25..49
                     /label=spacer from I12007 (1-11, 59-72)
     promoter        72..77
                     /label=-35
     promoter        95..100
                     /label=-10
     misc_feature    50..56
                     /label=spacer from I12007 (26-32)
     misc_feature    78..94
                     /label=spacer from I12007 (54-70)
     misc_feature    101..106
                     /label=spacer from I12007 (77-82)
     promoter        1..106
                     /label=BBa_J58100
     misc_feature    119..122
                     /label=conserved
     RBS             115..126
                     /label=BBa_B0034
     CDS             133..807
                     /label=mrfp1
     misc_feature    814..838
                     /label=Help:Barcodes
     CDS             133..813
                     /label=BBa_E1010
     stem_loop       858..901
                     /label=stem_loop
     misc_feature    847..926
                     /label=BBa_B0010
     terminator      847..926
                     /label=BBa_B0010
     stem_loop       942..961
                     /label=T7 TE
     misc_feature    968..968
                     /label=stop
     polyA_site      962..975
                     /label=polya
     misc_feature    935..975
                     /label=BBa_B0012
     terminator      935..975
                     /label=BBa_B0012
     misc_feature    115..975
                     /label=BBa_I13507
ORIGIN
        1 agaaatgtga tctagatcac atttgcaacc attataaata gtggtgataa aatagtcaac
       61 acgcacggtg ttagatattt ataaatagtg gtgatagatt taacgttact agagaaagag
      121 gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt
      181 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
      241 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
      301 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
      361 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
      421 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
      481 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
      541 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct
      601 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
      661 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
      721 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt
      781 gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta
      841 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      901 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      961 ctttctgcgt ttata
//