##gff-version 3
##sequence-region BBa_J58101 1 975
BBa_J58101	.	engineered_region	115	975	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_J58101	.	CDS	133	813	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_J58101	.	engineered_tag	814	838	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J58101	.	CDS	133	807	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J58101	.	terminator	847	926	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_J58101	.	engineered_region	847	926	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58101	.	stem_loop	858	901	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58101	.	ribosome_entry_site	115	126	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_J58101	.	conserved_region	119	122	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58101	.	terminator	935	975	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_J58101	.	stop_codon	968	968	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58101	.	polyA_site	962	975	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58101	.	stem_loop	942	961	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58101	.	engineered_region	935	975	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58101	.	promoter	1	106	.	+	0	ID=BBa_J58100;Name=BBa_J58100
BBa_J58101	.	promoter	95	100	.	+	0	ID=annotation1908179;Name=-10;Parent=BBa_J58100
BBa_J58101	.	promoter	72	77	.	+	0	ID=annotation1908178;Name=-35;Parent=BBa_J58100
BBa_J58101	.	sequence_feature	101	106	.	+	0	ID=annotation1908182;Name=spacer from I12007 (77-82);Parent=BBa_J58100
BBa_J58101	.	sequence_feature	50	56	.	+	0	ID=annotation1908180;Name=spacer from I12007 (26-32);Parent=BBa_J58100
BBa_J58101	.	operator	3	24	.	+	0	ID=annotation1908168;Name=CRP binding site;Parent=BBa_J58100
BBa_J58101	.	sequence_feature	78	94	.	+	0	ID=annotation1908181;Name=spacer from I12007 (54-70);Parent=BBa_J58100
BBa_J58101	.	sequence_feature	25	49	.	+	0	ID=annotation1908171;Name=spacer from I12007 (1-11, 59-72);Parent=BBa_J58100
BBa_J58101	.	operator	57	74	.	+	0	ID=annotation1908169;Name=lam-cI OR2 binding site;Parent=BBa_J58100
>BBa_J58101
agaaatgtgatctagatcacatttgcaaccattataaatagtggtgataaaatagtcaacacgcacggtgttagatatt
tataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaa
agagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggt
cgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgt
ccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttccc
ggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaa
gacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaacca
tgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaact
gaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttac
aaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtc
gtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata