LOCUS       BBa_J58110              1254 bp    DNA     linear       08-May-2024
DEFINITION  Synthetic vanillin receptor regulated by LacI
ACCESSION   BBa_J58110
VERSION     BBa_J58110.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1117
                     /label=BBa_J58105
     stem_loop       1137..1180
                     /label=stem_loop
     misc_feature    1126..1205
                     /label=BBa_B0010
     terminator      1126..1205
                     /label=BBa_B0010
     stem_loop       1221..1240
                     /label=T7 TE
     misc_feature    1247..1247
                     /label=stop
     polyA_site      1241..1254
                     /label=polya
     misc_feature    1214..1254
                     /label=BBa_B0012
     terminator      1214..1254
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acatgaaaaa
      241 actggctacc ctggtttccg ctgttgcgct aagcgccacc gtcagtgcga atgcgatggc
      301 aaaagacacc atcgcgctgg tggtcgaaac gcttaacaaa ccggacaacg tatcgctgaa
      361 agatggcgcg cagaaagagg cggataaact tggctataac ctggtggtgc tggactccca
      421 gaacaacccg gcgaaagagc tggcgaacgt gcaggactta accgttcgcg gcacaaaaat
      481 tctgctgatt gttccgaccg actccgacgc agtgggtaat gctgtgaaga tggctaacca
      541 ggcgaacatc ccggttatca ctcttaaacg ccaggcaacg aaaggtgaag tggtgagcca
      601 cattgctgcg gataacgtac tgggcggcaa aatcgctggt gattacatcg cgaagaaagc
      661 gggtgaaggt gccaaagtta tcgagctgca aggcaaagct ggtacatccg cagcccgtga
      721 actgggcgaa ggcttccagc aggccgttgc tgctcacaag tttaatgttc ttgccagcca
      781 gccagcagat gaagatcgca ttaaaggttt gaacgtaatg cagaacctgt tgaccgctca
      841 tccggatgtt caggctgtat tcgcgcagca ggatgaaatg gcgctgggcg cgctgcgcgc
      901 actgcaaact gccggtaaat cggatgtgat ggtcgtcgga gacgttggta caccggatgg
      961 cgaaaaagcg gtgaatgatg gcaaactagc agcgactatc gctgaactac ccgatcagat
     1021 tggcgcgaaa ggcgtcgaaa ccgcagataa agtgctgaaa ggcgagaaag ttcaggctaa
     1081 gtatccggtt gatctgaaac tggttgttaa gcagtagtac tagagccagg catcaaataa
     1141 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1201 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//