##gff-version 3 ##sequence-region BBa_J58110 1 1254 BBa_J58110 . CDS 227 1117 . + 0 ID=BBa_J58105;Name=BBa_J58105 BBa_J58110 . terminator 1126 1205 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J58110 . stem_loop 1137 1180 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J58110 . engineered_region 1126 1205 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J58110 . terminator 1214 1254 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J58110 . polyA_site 1241 1254 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J58110 . engineered_region 1214 1254 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J58110 . stem_loop 1221 1240 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J58110 . stop_codon 1247 1247 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J58110 . ribosome_entry_site 209 220 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J58110 . conserved_region 213 216 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J58110 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_J58110 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_J58110 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_J58110 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_J58110 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_J58110 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_J58110 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_J58110 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 >BBa_J58110 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaacatgaa aaaactggctaccctggtttccgctgttgcgctaagcgccaccgtcagtgcgaatgcgatggcaaaagacaccatcgcg ctggtggtcgaaacgcttaacaaaccggacaacgtatcgctgaaagatggcgcgcagaaagaggcggataaacttggct ataacctggtggtgctggactcccagaacaacccggcgaaagagctggcgaacgtgcaggacttaaccgttcgcggcac aaaaattctgctgattgttccgaccgactccgacgcagtgggtaatgctgtgaagatggctaaccaggcgaacatcccg gttatcactcttaaacgccaggcaacgaaaggtgaagtggtgagccacattgctgcggataacgtactgggcggcaaaa tcgctggtgattacatcgcgaagaaagcgggtgaaggtgccaaagttatcgagctgcaaggcaaagctggtacatccgc agcccgtgaactgggcgaaggcttccagcaggccgttgctgctcacaagtttaatgttcttgccagccagccagcagat gaagatcgcattaaaggtttgaacgtaatgcagaacctgttgaccgctcatccggatgttcaggctgtattcgcgcagc aggatgaaatggcgctgggcgcgctgcgcgcactgcaaactgccggtaaatcggatgtgatggtcgtcggagacgttgg tacaccggatggcgaaaaagcggtgaatgatggcaaactagcagcgactatcgctgaactacccgatcagattggcgcg aaaggcgtcgaaaccgcagataaagtgctgaaaggcgagaaagttcaggctaagtatccggttgatctgaaactggttg ttaagcagtagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata