LOCUS       BBa_J58111              1848 bp    DNA     linear       08-May-2024
DEFINITION  Trg-EnvZ regulated by LacI
ACCESSION   BBa_J58111
VERSION     BBa_J58111.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1711
                     /label=BBa_J58104
     stem_loop       1731..1774
                     /label=stem_loop
     misc_feature    1720..1799
                     /label=BBa_B0010
     terminator      1720..1799
                     /label=BBa_B0010
     stem_loop       1815..1834
                     /label=T7 TE
     misc_feature    1841..1841
                     /label=stop
     polyA_site      1835..1848
                     /label=polya
     misc_feature    1808..1848
                     /label=BBa_B0012
     terminator      1808..1848
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga atacaactcc
      241 ctcacagcga ttaggttttt tgcatcacat caggttggtt ccgttatttg cctgcattct
      301 aggcggtatc ttagttctat tcgcattaag ttcagccctg gctggctatt tcctctggca
      361 ggccgatcgc gatcagcgtg atgttactgc ggagattgag attcggaccg ggttagcgaa
      421 cagttcagat tttttgcgtt cagcccggat caatatgatt caggccgggg ctgcgagtcg
      481 tattgcggaa atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca
      541 gtcgcagcaa ggttatcgtg cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc
      601 cctcgacact gaattaaatc aacgctttca ggcttatatc acgggtatgc aacctatgtt
      661 gaaatatgcc aaaaatggca tgtttgaagc gattatcaat catgaaagtg agcagatccg
      721 accgctggat aatgcttata ccgatatttt gaacaaagcc gttaagatac gtagcaccag
      781 agccaaccaa ctggcggaac tggcccatca gcgcacccgc ctgggtggga tgttcatgat
      841 tggcgcgttt gtgcttgccc tggtcatgac gctgataaca tttatggtgc tacgtcggat
      901 cgtcattcgt ccactgcaac atgccgcaca acggattgaa aaaatcgcca gtggcgatct
      961 gacgatgaat gatgaaccgg cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca
     1021 gcatatggcg gctggtgtta agcaactggc ggatgaccgc acgctgctga tggcgggggt
     1081 aagtcacgac ttgcgcacgc cgctgacgcg tattcgcctg gcgactgaga tgatgagcga
     1141 gcaggatggc tatctggcag aatcgatcaa taaagatatc gaagagtgca acgccatcat
     1201 tgagcagttt atcgactacc tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct
     1261 taatgcagta ctcggtgagg tgattgctgc cgaaagtggc tatgagcggg aaattgaaac
     1321 cgcgctttac cccggcagca ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt
     1381 ggcgaatatg gtggtcaacg ccgcccgtta tggcaatggc tggatcaaag tcagcagcgg
     1441 aacggagccg aatcgcgcct ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga
     1501 acaacgtaag cacctgttcc agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg
     1561 cacgggatta gggctggcaa ttgtgcagcg tatcgtggat aaccataacg ggatgctgga
     1621 gcttggcacc agcgagcggg gcgggctttc cattcgcgcc tggctgccag tgccggtaac
     1681 gcgggcgcag ggcacgacaa aagaagggta atactagagc caggcatcaa ataaaacgaa
     1741 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1801 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//